Schuster G S, Erbland J F, Wyrick S D, Singh B B
Department of Oral Biology, Medical College of Georgia, School of Dentistry, Augusta 30912-0200.
Cytobios. 1988;54(216):53-60.
Although the specific mechanisms by which phorbol ester tumour promoters exert their various effects are not known, their actions are mediated by cell membrane receptors which contain lipids as major components of the receptor complex. Since cell modulators such as retinoic acid (RA) and nitrosonornicotine (NNN) can alter cell lipids, the binding of a phorbol ester to cells was examined at time intervals when lipid changes mediated by these modulators occur. Epithelial cells prepared from hamster cheek pouches were treated with all-trans RA or NNN for varying periods of time, then specific binding of phorbol esters was investigated. Cells treated with RA for intervals up to 24 h showed decreased binding when compared with untreated cells. Those treated with NNN for up to 168 h showed increased specific binding. The results suggest that alterations in the cell lipids may affect the specific binding of phorbol esters to cells.
虽然佛波酯肿瘤启动子发挥其各种作用的具体机制尚不清楚，但其作用是由细胞膜受体介导的，这些受体含有脂质作为受体复合物的主要成分。由于细胞调节剂如视黄酸（RA）和亚硝基去甲烟碱（NNN）可以改变细胞脂质，因此在由这些调节剂介导的脂质变化发生的时间间隔内，检测了佛波酯与细胞的结合情况。用全反式视黄酸或亚硝基去甲烟碱处理不同时间段的仓鼠颊囊上皮细胞，然后研究佛波酯的特异性结合。与未处理的细胞相比，用视黄酸处理长达24小时的细胞显示结合减少。用亚硝基去甲烟碱处理长达168小时的细胞显示特异性结合增加。结果表明，细胞脂质的改变可能会影响佛波酯与细胞的特异性结合。
