Žiarovská Jana, Medo Juraj, Kyseľ Matúš, Zamiešková Lucia, Kačániová Miroslava
Department of Genetics and Plant Breeding, Faculty of Agrobiology and Food Resources, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Department of Microbiology, Faculty of Biotechnology and Food Sciences, Slovak University of Agriculture in Nitra, Tr. A. Hlinku 2, 94976 Nitra, Slovakia.
Plants (Basel). 2020 Feb 18;9(2):266. doi: 10.3390/plants9020266.
Endophytic bacteria are an important part of different functions in plants that lead to plants' production characteristics as well as their stress response mechanisms. Endophytic bacterial diversity was analyzed in this study to describe 16S rRNA variability and changes in the leaves of drought-tolerant and drought-susceptible wheat when growth under in vitro conditions. A metagenomic analysis was applied and a pilot exploratory study was performed to prove this type of analysis as applicable to tracking endophytic bacterial diversity changes when a drought stress is applied to an in vitro culture of wheat. The study showed that the changes in the bacterial endophytes' variabilities associated preferentially with the drought stress varietal characteristics of the analyzed wheat instead of the applied stress conditions.
内生细菌是植物不同功能的重要组成部分,这些功能会影响植物的生产特性及其应激反应机制。本研究分析了内生细菌多样性,以描述在体外条件下生长时,耐旱和易旱小麦叶片中的16S rRNA变异性及变化情况。应用了宏基因组分析并进行了初步探索性研究,以证明这种分析方法适用于追踪对小麦体外培养施加干旱胁迫时内生细菌多样性的变化。研究表明,内生细菌变异性的变化优先与所分析小麦的干旱胁迫品种特性相关,而非与施加的胁迫条件相关。