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CRISPR/Cas9介导的[具体物种]中[具体基因]的诱变。 (你提供的原文不完整,这里是根据格式推测补充完整后的翻译,你可根据实际情况调整)

CRISPR/Cas9-Mediated Mutagenesis of in .

作者信息

Alvim Kamei Claire Lessa, Pieper Bjorn, Laurent Stefan, Tsiantis Miltos, Huijser Peter

机构信息

Department of Comparative Development and Genetics, Max Planck Institute for Plant Breeding Research, Carl-von-Linné-Weg 10, 50829 Cologne, Germany.

Hudson River Biotechnology, Nieuwe Kanaal 7V, 6709 PA Wageningen, The Netherlands.

出版信息

Plants (Basel). 2020 Feb 18;9(2):268. doi: 10.3390/plants9020268.

DOI:10.3390/plants9020268
PMID:32085527
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7076481/
Abstract

The small crucifer bears complex leaves divided into leaflets. This is in contrast to its relative, the reference plant , which has simple leaves. Comparative studies between these species provide attractive opportunities to study the diversification of form. Here, we report on the implementation of the CRISPR/Cas9 genome editing methodology in and with it the generation of novel alleles in the gene, which was previously shown to play a major role in the diversification of form between the two species. Thus, genome editing can now be deployed in , thereby increasing its versatility as a model system to study gene function and evolution.

摘要

这种小型十字花科植物长有复杂的叶子,这些叶子分裂成小叶。这与其近缘参考植物形成对比,后者具有单叶。对这些物种进行比较研究,为研究形态多样化提供了有吸引力的机会。在这里,我们报告了CRISPR/Cas9基因组编辑方法在[物种名称]中的应用,以及利用该方法在[基因名称]基因中产生的新等位基因,先前的研究表明该基因在这两个物种的形态多样化中起主要作用。因此,现在可以在[物种名称]中应用基因组编辑技术,从而增加其作为研究基因功能和进化的模型系统的通用性。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/b2702687e742/plants-09-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/4727d06f3669/plants-09-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/6eb11b35db66/plants-09-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/e9a341a2b891/plants-09-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/b2702687e742/plants-09-00268-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/4727d06f3669/plants-09-00268-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/6eb11b35db66/plants-09-00268-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/e9a341a2b891/plants-09-00268-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ffb6/7076481/b2702687e742/plants-09-00268-g004.jpg

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本文引用的文献

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Resolving the backbone of the Brassicaceae phylogeny for investigating trait diversity.解析芸薹科系统发育的骨干,以研究性状多样性。
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Coupled enhancer and coding sequence evolution of a homeobox gene shaped leaf diversity.同源异型盒基因的增强子与编码序列协同进化塑造了叶片多样性。
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The Cardamine hirsuta genome offers insight into the evolution of morphological diversity.长角山荷叶基因组揭示了形态多样性的进化。
Nat Plants. 2016 Oct 31;2(11):16167. doi: 10.1038/nplants.2016.167.
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Curr Opin Genet Dev. 2016 Aug;39:1-7. doi: 10.1016/j.gde.2016.05.005. Epub 2016 Jun 5.
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Morphomechanical Innovation Drives Explosive Seed Dispersal.形态力学创新推动种子的爆发式传播。
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