National Centre for Cell Science, NCCS Complex, SP Pune University Campus, Pune, Maharashtra, India.
Adv Protein Chem Struct Biol. 2020;120:409-424. doi: 10.1016/bs.apcsb.2019.12.004. Epub 2020 Jan 10.
The complement system is one of the first barriers and consists of well-balanced cascades of reactions which generates anaphylatoxins such as C5a and C3a. A G-protein coupled receptor C5a anaphylatoxin chemotactic receptor 1 (C5AR1, also known as CD88) is the receptor for C5a which is present on cells of myeloid origin. Owing to difficulty in obtaining crystal structures of GPCRs in either inactive or active state, accurate structural modeling is still highly desirable for the majority of GPCRs. In an attempt to dissect the conformational changes associated with GPCR activation, computational modeling approaches is being pursued in this paper along with the evolutionary divergence to deal with the structural variability.
补体系统是第一道屏障之一，由一系列平衡反应级联组成，产生过敏毒素，如 C5a 和 C3a。G 蛋白偶联受体 C5a 过敏毒素趋化受体 1（C5AR1，也称为 CD88）是 C5a 的受体，存在于髓系细胞上。由于难以获得非活性或活性状态下 GPCR 的晶体结构，因此对于大多数 GPCR 而言，准确的结构建模仍然是非常需要的。在本文中，我们尝试通过计算建模方法来剖析与 GPCR 激活相关的构象变化，同时考虑进化分歧来处理结构变异性。
