Laboratório de Protistologia, Departamento de Zoologia, Instituto de Biologia, CCS, Universidade Federal do Rio de Janeiro (UFRJ), Ilha do Fundão, Rio de Janeiro, Brazil.
Laboratório de Protistologia, Departamento de Zoologia, Instituto de Biologia, CCS, Universidade Federal do Rio de Janeiro (UFRJ), Ilha do Fundão, Rio de Janeiro, Brazil.
Protist. 2020 Apr;171(2):125716. doi: 10.1016/j.protis.2020.125716. Epub 2020 Feb 7.
The genus Parablepharisma Jankowski, 2007 at present includes five species, most of which have not been studied in detail, therefore phylogenetic affinities remained uninvestigated up to now. Parablepharisma is traditionally placed within Heterotrichea based on insufficient existing morphological data, and there are no available Parablepharisma gene sequences in molecular databases to support this placement. This work presents an 18S rDNA-based phylogeny of P. bacteriophora (Kahl, 1932) Jankowski, 2007 and P. brasiliensis sp. nov. We also provide a redescription of P. bacteriophora and P. chlamydophorum (Kahl, 1932) Jankowski, 2007 based on live morphological observations combined with silver impregnation and scanning and transmission electron microscopy. According to characters such as macro- and micronucleus number, the position of the ectosymbiotic bacteria, and the presence/absence of caudal cilia, two new species are described, i.e. P. granulata sp. nov. and P. brasiliensis sp. nov. In addition, we establish Kahlium gen. nov. to include P. chlamydophorum, which has a segmented anterior paroral portion and a twisted posterior paroral section as diagnostic features. To include Parablepharisma and Kahlium gen. nov., we propose Parablepharismidae fam. nov. According to our phylogenetic analyses, Parablepharisma belongs to SAL (Spirotrichea, Armophorea, Litostomatea), being a sister group of Cariacotrichea.
目前 Parablepharisma Jankowski, 2007 属包括五个物种，其中大多数尚未详细研究，因此到目前为止尚未研究其系统发育关系。Parablepharisma 传统上基于现有形态学数据不足被置于 Heterotrichea 中，并且在分子数据库中没有可用的 Parablepharisma 基因序列来支持这种分类。本工作基于 18S rDNA 对 P. bacteriophora (Kahl, 1932) Jankowski, 2007 和 P. brasiliensis sp. nov. 的系统发育进行了研究。我们还根据活体形态观察、银染和扫描及透射电子显微镜对 P. bacteriophora 和 P. chlamydophorum (Kahl, 1932) Jankowski, 2007 进行了重新描述。根据大核和小核数量、共生细菌的位置以及尾纤毛的存在与否等特征，描述了两个新物种，即 P. granulata sp. nov. 和 P. brasiliensis sp. nov. 此外，我们建立 Kahlium gen. nov. 以包含 P. chlamydophorum，其具有分段的前口环和扭曲的后口环部分作为诊断特征。为了包含 Parablepharisma 和 Kahlium gen. nov.，我们提出 Parablepharismidae fam. nov. 根据我们的系统发育分析，Parablepharisma 属于 SAL（旋毛目、臂毛目、Litostomatea），是 Cariacotrichea 的姊妹群。
