Ollmann M, Robitzki A, Schwarzmann G, Galla H J
Institut für Biochemie, Technische Hochschule Darmstadt, Federal Republic of Germany.
Eur Biophys J. 1988;16(2):109-12. doi: 10.1007/BF00255520.
We have investigated the effect of bulk viscosity on lipid translational diffusion using the excimer formation technique. In contrast to a study by Vaz et al. (1987), performed with the fluorescence recovery after photobleaching technique, we observed only a minor decrease of less than a factor of two for pyrene labelled phosphatidylcholine in glycerinated phosphatidylcholine bilayer membranes compared to an aqueous dispersion. Even the diffusion of pyrene labelled gangliosides with an oligosaccharide head-group that protrudes from the membrane surface is not strongly restricted by the increased bulk viscosity. We conclude that the viscosity of the fluid bounding the lipid bilayers is of minor importance for the diffusion of membrane lipids.
我们使用准分子形成技术研究了体相粘度对脂质平移扩散的影响。与Vaz等人（1987年）使用光漂白后荧光恢复技术进行的研究不同，我们观察到，与水分散体相比，在甘油化磷脂酰胆碱双层膜中，芘标记的磷脂酰胆碱的扩散仅略有下降，下降幅度不到两倍。即使是带有从膜表面突出的寡糖头部基团的芘标记神经节苷脂的扩散，也不会受到体相粘度增加的强烈限制。我们得出结论，包围脂质双层的流体的粘度对膜脂质的扩散影响较小。
