Müller H J, Galla H J
Biochim Biophys Acta. 1983 Sep 7;733(2):291-4. doi: 10.1016/0005-2736(83)90535-7.
A pressure-induced decrease of the lateral diffusion in pure and cholesterol containing phosphatidylcholine bilayer membranes has been determined by the excimer formation technique using pyrene as probe molecule. The experimental results at pressures up to 150 bars are described satisfactorily by the free volume theory of a molecular transport in liquids. A pressure increase of extrapolated 575 bars decreases the lateral diffusion of lipids by a factor of two in pure dipalmitoylphosphatidylcholine membranes. Higher pressures are necessary to induce the same effect in cholesterol containing membranes. This result is interpreted by the condensing effect of cholesterol in fluid bilayer membranes.
使用芘作为探针分子，通过准分子形成技术测定了压力诱导的纯磷脂酰胆碱双层膜和含胆固醇的磷脂酰胆碱双层膜中横向扩散的降低。液体中分子传输的自由体积理论令人满意地描述了高达150巴压力下的实验结果。在纯二棕榈酰磷脂酰胆碱膜中，外推压力增加575巴会使脂质的横向扩散降低两倍。在含胆固醇的膜中需要更高的压力才能产生相同的效果。这一结果可通过胆固醇在流体双层膜中的凝聚作用来解释。
