Affiliated Hospital of Hebei Engineering University, Hebei, Handan, China.
Handan Municipal Center for Disease Control and Prevention, Hebei, Handan, China.
Ecotoxicol Environ Saf. 2020 Apr 15;193:110302. doi: 10.1016/j.ecoenv.2020.110302. Epub 2020 Feb 19.
Based on previous studies and preliminary test results, 200 μM was used as the test concentration of chromium (Cr), and changes in the gene expression profile of Arabidopsis thaliana in response to 24-h treatments of Cr(III) and Cr(VI) were analyzed using the Arabidopsis ATH1 Genome Array. The results were as follows. There were 238 upregulated genes and 858 downregulated genes in response to treatments with Cr(III) and Cr(VI). For Cr(III) and Cr(VI) treatments, there were 185 and 587 specifically upregulated genes as well as 220 and 956 specifically downregulated genes, respectively. Among the common differentially expressed genes (DEGs), the expression levels of genes involved in redox, secondary metabolism, and energy metabolism processes were significantly downregulated, while those of genes related to the stress response, photosynthesis, and sulfur metabolism were significantly upregulated. These findings indicated that Cr seriously affected the normal activities of A. thaliana cells. Some genes associated with stress and regulation were upregulated to adapt to the stress caused by Cr. Among the unique DEGs, the expression levels of genes involved in indole-3-acetic acid (IAA) regulatory pathway were significantly increased in response to Cr(III) treatment; the expression levels of genes involved in the abscisic acid (ABA) regulation pathway and carotenoid synthesis were significantly increased following Cr(VI) treatment. These results revealed some differences in response to Cr(III) and Cr(VI) in A. thaliana.
基于先前的研究和初步的测试结果，将 200μM 用作铬（Cr）的测试浓度，使用拟南芥 ATH1 基因组阵列分析了 Cr(III)和 Cr(VI) 24 小时处理后拟南芥基因表达谱的变化。结果如下。Cr(III)和 Cr(VI)处理分别有 238 个上调基因和 858 个下调基因。对于 Cr(III)和 Cr(VI)处理，分别有 185 个和 587 个特异性上调基因以及 220 个和 956 个特异性下调基因。在共同差异表达基因（DEGs）中，参与氧化还原、次生代谢和能量代谢过程的基因表达水平显著下调，而与应激反应、光合作用和硫代谢相关的基因表达水平显著上调。这些发现表明 Cr 严重影响了拟南芥细胞的正常活动。一些与应激和调节相关的基因被上调以适应 Cr 引起的应激。在独特的 DEGs 中，参与吲哚-3-乙酸（IAA）调节途径的基因表达水平在 Cr(III)处理时显著增加；参与脱落酸（ABA）调节途径和类胡萝卜素合成的基因表达水平在 Cr(VI)处理时显著增加。这些结果揭示了拟南芥对 Cr(III)和 Cr(VI)的反应存在一些差异。
