College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China.
College of Environmental Science and Engineering, Ocean University of China, Qingdao 266100, China; Key Laboratory of Marine Environmental and Ecology, Ministry of Education, Ocean University of China, Qingdao 266100, China.
Bioresour Technol. 2020 May;304:122982. doi: 10.1016/j.biortech.2020.122982. Epub 2020 Feb 11.
Heterotrophic denitrification using mariculture solid wastes (MSW) fermentation liquid as carbon source is an economically and environmentally sustainable strategy for NO-N removal in marine recycling aquaculture systems (RAS). The optimization of COD/NO-N ratio (C/N) and hydraulic retention times (HRT) with respect to MSW fermentation liquid driven denitrification for marine RAS wastewater treatment was investigated. The optimum C/N of 8 and HRT of 6 h for heterotrophic denitrification was obtained with NO-N removal efficiency of 97.8% and 94.2%, respectively. Using MSW fermentation liquid as carbon source, the utilization of VFAs was more effective than that of carbohydrates and proteins, and effluent COD concentration decreased with an increment in HRT from 4 to 8 h. The results of high-throughput sequencing analysis showed microbial communities were enriched selectively in the reactors by optimizing C/N and HRT, which obviously enhanced the nitrogen removal in respect to MSW fermentation liquid driven denitrification.
利用海水养殖固体废弃物（MSW）发酵液作为碳源进行异养反硝化是一种经济且环境可持续的方法，可用于去除海洋循环水产养殖系统（RAS）中的硝态氮（NO-N）。本研究旨在探讨 COD/NO-N 比（C/N）和水力停留时间（HRT）优化对 MSW 发酵液驱动反硝化处理海水 RAS 废水的影响。结果表明，当 C/N 为 8、HRT 为 6 h 时，反硝化的最佳条件为 97.8%和 94.2%的 NO-N 去除效率。利用 MSW 发酵液作为碳源时，VFAs 的利用率比碳水化合物和蛋白质更有效，并且随着 HRT 从 4 小时增加到 8 小时，出水 COD 浓度逐渐降低。高通量测序分析的结果表明，通过优化 C/N 和 HRT，选择性地富集了反应器中的微生物群落，这明显增强了 MSW 发酵液驱动反硝化中的氮去除。
