贝酸钙负载碳酸钙微颗粒通过提高线粒体活性和降低活性氧来提高玻璃化冷冻猫腔前卵泡的存活率。
Betaine-loaded CaCO microparticles improve survival of vitrified feline preantral follicles through higher mitochondrial activity and decreased reactive oxygen species.
机构信息
Laboratory of Cytogenetics, Center for Advanced Studies in Biodiversity, Biological Sciences Institute, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil; and Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil; and Laboratory of Manipulation of Oocytes and Ovarian Pre-Antral Follicles, Faculty of Veterinary Medicine, Ceará State University, Av. Dr. Silas Munguba, 1700 - Itaperi, 60714-903, Fortaleza-CE, Brazil; and Corresponding author. Email:
Laboratory of Wild Animal Biology and Medicine, Federal University of Pará, Av. Perimetral, 2-224 - Guamá, 66077-830, Belém-PA, Brazil.
出版信息
Reprod Fertil Dev. 2020 Mar;32(5):531-537. doi: 10.1071/RD19068.
Ovary fragments from six sexually mature cats were vitrified in the presence or absence of betaine or ascorbic acid, loaded (7.4 or 74µM betaine; 20 or 200µM ascorbic acid) or not (1mM betaine or 0.3mM ascorbic acid) into CaCO3 microparticles, and assessed for follicular morphology, oxidative stress and mitochondrial activity Feline ovarian tissue was successfully preserved after vitrification in the presence of 74µM betaine loaded in CaCO3 microparticles, as confirmed by morphological analysis and the density of preantral follicles and stromal cells, as well as by the increased mitochondrial activity and decreased production of reactive oxygen species.
从 6 只性成熟的猫中取出的卵巢碎片在有或没有甜菜碱或抗坏血酸的情况下进行玻璃化处理，装载（7.4 或 74µM 甜菜碱；20 或 200µM 抗坏血酸）或不装载（1mM 甜菜碱或 0.3mM 抗坏血酸）到 CaCO3 微颗粒中，并评估卵泡形态、氧化应激和线粒体活性。经证实，在 CaCO3 微颗粒中装载 74µM 甜菜碱的情况下，猫的卵巢组织在玻璃化处理后成功保存，这可以通过形态分析以及原始卵泡和基质细胞的密度、线粒体活性的增加和活性氧产生的减少来确认。
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