Goltermann Lise, Nielsen Peter E
Department of Cellular and Molecular Medicine, Faculty of Health and Medical Sciences, University of Copenhagen, Copenhagen, Denmark.
Methods Mol Biol. 2020;2105:231-239. doi: 10.1007/978-1-0716-0243-0_14.
Antisense PNA-peptide conjugates targeting essential bacterial genes have shown interesting potential for discovery of novel precision antibiotics. In this context, the minimal inhibitory concentration (MIC) assay is used to assess and compare the antimicrobial activity of natural as well as synthetic antimicrobial compounds. Here, we describe the determination of the minimal inhibitory concentration of peptide-PNA conjugates against Escherichia coli. This method can be expanded to include minimal bactericidal concentration (MBC) determination and kill-curve kinetics.
靶向细菌必需基因的反义肽核酸（PNA）缀合物在发现新型精准抗生素方面展现出了有趣的潜力。在此背景下，最低抑菌浓度（MIC）测定法用于评估和比较天然及合成抗菌化合物的抗菌活性。在此，我们描述了肽 - PNA缀合物对大肠杆菌最低抑菌浓度的测定方法。该方法可扩展至包括最低杀菌浓度（MBC）测定和杀菌曲线动力学研究。
