el Moataz Bellah M, el Ridi R, Abou-Elela R, Cooper E L
Entomology Department, Faculty of Science, Cairo University, Egypt.
Dev Comp Immunol. 1988 Fall;12(4):707-17. doi: 10.1016/0145-305x(88)90046-8.
Natural agglutinins against human, guinea pig, and rat erythrocytes (RBC) and bacteria Bacillus thuringiensis were readily detected in hemolymph of final instar larvae with resulting titers of 0-9 (log2). Titers were independent of sex and season but varied conspicuously and reproducibly with age. Moreover, response of hemagglutinins to heating varied with age being heat-labile in younger larvae, yet totally resistant to heating for 30 min at 70 degrees C in older ones. Bacterial agglutinins were uniformly resistant at all larval ages. The study thus reveals that the amounts and physico-chemical properties of P. ricini agglutinins change with larval development. Therefore, larval age should be taken into close consideration before reporting on the occurrence and properties of agglutinins in insects.
在末龄幼虫的血淋巴中很容易检测到针对人、豚鼠和大鼠红细胞(RBC)以及苏云金芽孢杆菌的天然凝集素,其效价为0 - 9(log2)。效价与性别和季节无关,但随年龄显著且可重复地变化。此外,血凝素对加热的反应随年龄而变化,较年轻的幼虫中血凝素对热不稳定,而在较年长的幼虫中,在70摄氏度下加热30分钟则完全耐热。在所有幼虫年龄段,细菌凝集素均具有抗性。因此,该研究表明蓖麻蚕凝集素的数量和理化性质随幼虫发育而变化。因此,在报告昆虫凝集素的存在和性质之前,应密切考虑幼虫的年龄。