Le Magueresse B, Jégou B
Université de Rennes I, UA CNRS 256, France.
Mol Cell Endocrinol. 1988 Jul;58(1):65-72. doi: 10.1016/0303-7207(88)90054-8.
Enriched populations of germ cells prepared from adult rats were found to influence 20-day-old rat Sertoli cell secretory activity by stimulating androgen-binding protein (ABP) and inhibiting oestradiol-17 beta production in the presence of follicle-stimulating hormone (FSH) as well as of dibutyryl cyclic AMP (dbcAMP). Among the different populations tested in coculture, pachytene spermatocytes were the most effective at stimulating ABP and inhibiting oestradiol production, whereas early spermatids had relatively less effects. Cytoplasts from elongated spermatids only slightly stimulated ABP secretion. The influence of germ cells upon Sertoli cells may be mediated via paracrine component(s) detected in nonconcentrated conditioned culture media. The stimulatory (ABP) and inhibitory (oestradiol) effects of pachytene spermatocyte and early spermatid-spent media were reversible (change of media), dose related, specific (no effect of cytoplast, peritubular cell, rat liver epithelial cell or 3T3 cell-conditioned media) and strictly proportional to the cell viability estimated at the end of the incubation periods. Furthermore, the nature of the germ cell factor(s) influencing Sertoli cell secretory function is likely to be proteinaceous since both germ cell-spent media effects were trypsin and heat (100 degrees C; 3 min) sensitive and retained by molecular weight (MW) greater than 10,000 cut-off dialysis membranes. It is hypothesized that germ cells, in particular pachytene spermatocytes and early spermatids, may influence Sertoli cell function during sexual development in the rat.
研究发现，从成年大鼠制备的富集生殖细胞群体，在促卵泡激素（FSH）以及二丁酰环磷腺苷（dbcAMP）存在的情况下，通过刺激雄激素结合蛋白（ABP）和抑制雌二醇-17β的产生，来影响20日龄大鼠支持细胞的分泌活性。在共培养中测试的不同细胞群体中，粗线期精母细胞在刺激ABP和抑制雌二醇产生方面最有效，而早期精子细胞的作用相对较小。伸长型精子细胞的胞质体仅轻微刺激ABP分泌。生殖细胞对支持细胞的影响可能是通过在未浓缩的条件培养基中检测到的旁分泌成分介导的。粗线期精母细胞和早期精子细胞用过的培养基的刺激作用（ABP）和抑制作用（雌二醇）是可逆的（更换培养基）、剂量相关、具有特异性（胞质体、睾丸间质细胞、大鼠肝上皮细胞或3T3细胞条件培养基无作用），并且与孵育期结束时估计的细胞活力严格成比例。此外，影响支持细胞分泌功能的生殖细胞因子的性质可能是蛋白质，因为生殖细胞用过的培养基的两种作用都对胰蛋白酶和热（100℃；3分钟）敏感，并且能被截留分子量大于10,000的透析膜保留。据推测，生殖细胞，特别是粗线期精母细胞和早期精子细胞，可能在大鼠性发育过程中影响支持细胞的功能。
