吲哚美辛与前列腺素E2在大鼠脂肪细胞中的抗脂解作用的相互作用。

Interaction of indomethacin with the antilipolytic effect of prostaglandin E2 in rat adipocytes.

作者信息

Richelsen B

机构信息

Medical Department III, Aarhus Amtssygehus, Denmark.

出版信息

Mol Cell Endocrinol. 1988 Jul;58(1):85-91. doi: 10.1016/0303-7207(88)90056-1.

DOI:10.1016/0303-7207(88)90056-1

PMID:3208990

Abstract

The interaction of indomethacin with the antilipolytic effect of prostaglandin E2 (PGE2) was investigated in rat adipocytes in order to reveal a possible role of endogenous PGs in adipose tissue. Adipocytes isolated from rats treated in vivo for 5 days with indomethacin were compared with non-treated control rats. The sensitivity of the antilipolytic effect of exogenous PGE2 was significantly enhanced in adipocytes from indomethacin-treated rats (IC50 of PGE2: 0.45 +/- 0.05 nM vs. 1.2 +/- 0.1 nM, P less than 0.01). This enhanced antilipolytic effect of exogenous PGE2 could be related to the reduced endogenous PGE2 formation in adipocytes from the indomethacin-treated rats (PGE2 formation was reduced by more than 90%). In agreement with that observation the [p3H]PGE2 receptor binding was enhanced by 58% in adipocytes treated with indomethacin. Thus, indomethacin via inhibition of endogenous PG formation could modulate some properties of lipolysis in adipocytes. However, indomethacin treatment had no significant effects on basal or isoproterenol-stimulated lipolysis.

摘要

为了揭示内源性前列腺素（PGs）在脂肪组织中的可能作用，研究了吲哚美辛与前列腺素E2（PGE2）抗脂解作用之间的相互作用。将体内用吲哚美辛处理5天的大鼠分离出的脂肪细胞与未处理的对照大鼠进行比较。在吲哚美辛处理的大鼠的脂肪细胞中，外源性PGE2抗脂解作用的敏感性显著增强（PGE2的IC50：0.45±0.05 nM对1.2±0.1 nM，P<0.01）。外源性PGE2这种增强的抗脂解作用可能与吲哚美辛处理的大鼠脂肪细胞中内源性PGE2生成减少有关（PGE2生成减少超过90%）。与该观察结果一致，用吲哚美辛处理的脂肪细胞中，[p3H]PGE2受体结合增加了58%。因此，吲哚美辛通过抑制内源性PG生成可调节脂肪细胞中脂解的某些特性。然而，吲哚美辛处理对基础或异丙肾上腺素刺激的脂解没有显著影响。