College of Korean Medicine, Kyung Hee University, Seoul 02447, Republic of Korea.
Biomed Res Int. 2020 Feb 7;2020:5925094. doi: 10.1155/2020/5925094. eCollection 2020.
Saponins are a group of naturally occurring plant glycosides with the features of their strong foam-forming properties and multibiological effects such as antitumor activity. Though Misaponin B, one of the triterpenoid saponins from , is known to have spermicidal and antioxidant activity, the other biological activities have been never reported so far. Thus, in the present study, the antitumor mechanism of Misaponin B was investigated in A549 and AsPC-1 cancer cells. Misaponin B exerted significant cytotoxicity in A549, H460, SKOV3, and AsPC-1 cancer cells. Among them, A549 and AsPC-1 cells were more susceptible to Misaponin B. Misaponin B induced G2/M arrest and cytokinesis failure and increased the expression of LC3B and p62 with autophagic vacuoles and GFP-LC3 punctae in A549 and AsPC-1 cells. Furthermore, Misaponin B suppressed autophagy flux in A549 cells transfected by GFP-mRFP-LC3 constructs by showing merged yellow color by autophagy flux assay. Overall, our findings provide evidences that Misaponin B induces G2M arrest and impairs autophagy in A549 and AsPC-1 cells.
皂素是一组天然存在的植物糖苷，具有很强的泡沫形成特性和多种生物学效应，如抗肿瘤活性。虽然 中的三萜皂素之一——知母皂素 B 已知具有杀精和抗氧化活性，但迄今为止尚未报道其其他生物学活性。因此，在本研究中，研究了知母皂素 B 在 A549 和 AsPC-1 癌细胞中的抗肿瘤机制。知母皂素 B 在 A549、H460、SKOV3 和 AsPC-1 癌细胞中表现出显著的细胞毒性。其中，A549 和 AsPC-1 细胞对知母皂素 B 更敏感。知母皂素 B 诱导 G2/M 期阻滞和胞质分裂失败，并增加 A549 和 AsPC-1 细胞中 LC3B 和 p62 的表达，出现自噬空泡和 GFP-LC3 点状。此外，通过自噬流测定，知母皂素 B 抑制了 GFP-mRFP-LC3 构建体转染的 A549 细胞中的自噬流，显示出合并的黄色。总的来说，我们的研究结果提供了证据表明知母皂素 B 诱导 A549 和 AsPC-1 细胞中的 G2M 阻滞并损害自噬。
