Caudy M, Grell E H, Dambly-Chaudière C, Ghysen A, Jan L Y, Jan Y N
Howard Hughes Medical Institute, University of California, San Francisco 94143.
Genes Dev. 1988 Jul;2(7):843-52. doi: 10.1101/gad.2.7.843.
The daughterless (da) gene is known to have separate maternal and zygotic functions: Maternally supplied daughterless activity is required for proper sex determination and dosage compensation in female embryos, whereas loss of zygotically supplied da+ activity causes embryonic lethality in both male and female embryos. We have found that the zygotic da+ activity is necessary for neural development: The use of neuron-specific antibodies and beta-galactosidase-marked X chromosomes has revealed that in both male and female embryos deletions or strong mutations of the da gene remove all peripheral neurons and associated sensory structures without disrupting the epithelium from which they derive. Partial da+ function causes partial removal of peripheral neurons. Our results indicate that da+ is required for the formation of peripheral neurons and their associated sensory structures.
已知无女儿基因（da）具有独立的母体功能和合子功能：母体提供的无女儿活性对于雌性胚胎中正确的性别决定和剂量补偿是必需的，而合子提供的da+活性丧失会导致雄性和雌性胚胎出现胚胎致死性。我们发现合子da+活性对于神经发育是必需的：使用神经元特异性抗体和β-半乳糖苷酶标记的X染色体已表明，在雄性和雌性胚胎中，da基因的缺失或强突变会去除所有外周神经元和相关的感觉结构，而不会破坏它们所源自的上皮组织。部分da+功能会导致外周神经元部分缺失。我们的结果表明，da+对于外周神经元及其相关感觉结构的形成是必需的。
