Beijing Key Laboratory of Bioprocess, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China.
Beijing Key Laboratory of Bioprocess, Beijing Advanced Innovation Center for Soft Matter Science and Engineering, Beijing University of Chemical Technology, Beijing, 100029, PR China; Institute of Nano Biomedicine and Engineering, School of Electronic Information and Electrical Engineering, Shanghai Jiao Tong University, Shanghai, 200240, PR China.
J Environ Manage. 2020 May 15;262:110245. doi: 10.1016/j.jenvman.2020.110245. Epub 2020 Feb 20.
To reduce the instability of aerobic granular sludge (AGS) caused by high-strength anaerobic digestion wastewater, a strategy of increasing proportion of anaerobic digestion wastewater step-by-step was adopted in this study. High-performance stable AGSs were successfully cultivated with sequencing batch reactors by this strategy, which could efficiently treat high-strength anaerobic digestion wastewater with an influent chemical oxygen demand (COD) up to 5090 mg⋅L. After six phases of stepwise increasing COD loads, the sludge sizes increased from 0.5 mm to 1.5 mm, with the final mixed liquor suspended solids increased to 13,814 mg⋅L, and the final sludge volume index decreased to 15 mL⋅g. The extracellular polymeric substance (EPS), which is crucial to keep the stability of AGS, increased continuously from 85.1 mg⋅g SS to 307.8 mg⋅g SS with the increase of COD loads. Moreover, the removal efficiency of COD and TN could reach 92% and 87% for real high-strength anaerobic digestion wastewater treatment. The bacterial community analysis revealed that the family Rhodocyclaceae, Flavobacteriaceae, and Xanthomonadaceae were the major microbes of AGS, and were responsible for COD and TN removal, as well as EPS secretion. These findings may provide novel information and enrich AGS treatment of high-strength real wastewater.
为了降低高强度厌氧消化废水引起的好氧颗粒污泥 (AGS) 的不稳定性，本研究采用逐步提高厌氧消化废水比例的策略。通过该策略，采用序批式反应器成功培养出高性能稳定的 AGS，可有效处理进水化学需氧量（COD）高达 5090 mg⋅L 的高强度厌氧消化废水。经过六轮逐步提高 COD 负荷的阶段，污泥粒径从 0.5mm 增加到 1.5mm，最终混合液悬浮固体（MLSS）增加到 13814mg⋅L，最终污泥体积指数（SVI）降低到 15mL⋅g。对于实际的高强度厌氧消化废水处理，胞外聚合物（EPS），这是保持 AGS 稳定性的关键，随着 COD 负荷的增加，从 85.1mg⋅g SS 持续增加到 307.8mg⋅g SS。此外，COD 和 TN 的去除效率可分别达到 92%和 87%。细菌群落分析表明，Rhodocyclaceae、Flavobacteriaceae 和 Xanthomonadaceae 是 AGS 的主要微生物，负责 COD 和 TN 的去除以及 EPS 的分泌。这些发现可能为 AGS 处理高强度实际废水提供新的信息和丰富的内容。
