Department of Ecology, Environment and Evolution, La Trobe University, Melbourne, Victoria 3086, Australia.
Centre for Sustainable Tropical Fisheries and Aquaculture, James Cook University, Townsville, Queensland 4811, Australia.
J Proteome Res. 2020 Apr 3;19(4):1491-1501. doi: 10.1021/acs.jproteome.9b00738. Epub 2020 Mar 5.
Cephalopods are known to produce an extensive range of secretions including ink, mucus, and venom. Sepiadariidae, a family of small, benthic bobtail squids, are notable for the high volume of viscous slime they emit when stressed. One species, (striped pyjama squid), is covered with glands along the perimeter of the ventral mantle, and these structures are hypothesized to be the source of its slime. Using label-free quantitative proteomics, we analyzed five tissue types (dorsal and ventral mantle muscle, dorsal and ventral epithelium, and ventral mantle glands) and the slime from four individuals. In doing so, we were able to determine the relationship between the slime and the tissues as well as highlight proteins that were specifically identified within the slime and ventral mantle glands. A total of 28 proteins were identified to be highly enriched in slime, and these were composed of peptidases and protease inhibitors. Seven of these proteins contained predicted signal peptides, indicating classical secretion, with four proteins having no identifiable domains or similarity to any known proteins. The ventral mantle glands also appear to be the tissue with the closest overall proteomic composition to the slime; therefore, it is likely that the slime originates, at least in part, from these glands.
头足类动物已知会产生广泛的分泌物，包括墨汁、黏液和毒液。蛇尾蛸科是一种小型底栖的短尾鱿鱼，当它们受到压力时，会分泌出大量粘稠的黏液，这一点很突出。其中一种名为 （条纹睡衣鱿鱼）的物种，其腹套边缘覆盖着腺体，这些结构被假设是其黏液的来源。我们使用无标记定量蛋白质组学技术，分析了来自四个个体的五种组织类型（背、腹套肌肉、背、腹套上皮和腹套腺体）和黏液。通过这样做，我们能够确定黏液与组织之间的关系，并突出在黏液和腹套腺中特异性鉴定的蛋白质。共有 28 种蛋白质被鉴定为在黏液中高度富集，这些蛋白质由肽酶和蛋白酶抑制剂组成。其中 7 种蛋白质含有预测的信号肽，表明它们是经典分泌途径，其中 4 种蛋白质没有可识别的结构域或与任何已知蛋白质相似。腹套腺体似乎也是与黏液整体蛋白质组组成最接近的组织；因此，黏液很可能至少部分来源于这些腺体。
