Jiangxi Provincial Key Laboratory of Water Resources and Environment of Poyang Lake, Jiangxi Provincial Institute of Water Sciences, Nanchang, 330029, China.
State Key Lab of Urban and Regional Ecology, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China.
Water Res. 2020 May 1;174:115603. doi: 10.1016/j.watres.2020.115603. Epub 2020 Feb 14.
Sewage treatment plants (STPs) are significant reservoirs of antibiotic resistance genes (ARGs) and antibiotic-resistant bacteria (ARB). Municipal STPs (MSTPs) and industrial STPs (ISTPs) are the two most important STP types in cities. In this study, the ARGs, mobile genetic elements (MGEs), and bacterial communities of selected STPs, including two MSTPs and one ISTP, in the vicinity of Poyang Lake were comprehensively investigated through high-throughput qPCR and high-throughput Illumina sequencing. The results showed that the profiles of ARGs, MGEs and bacteria differed between the ISTP and the two MSTPs, most likely due to differences in influent water quality, such as the Pb that characterized in the ISTP's influent. The longer hydraulic retention times (HRTs) of the two MSTPs than of the ISTP may also have accounted for the different profiles. Thus, a prolonged HRT in the CASS process seems to allow a more extensive removal of ARGs and bacteria in ISTPs with similar treatment process. By providing comprehensive insights into the characteristics of ARGs, MGEs and the bacterial communities of the selected MSTPs and ISTP, our study provides a scientific basis for controlling the propagation and diffusion of ARGs and ARB in different types of STPs.
污水处理厂（STPs）是抗生素耐药基因（ARGs）和抗生素耐药菌（ARB）的重要储存库。城市中的污水处理厂主要有两种类型，即市政污水处理厂（MSTPs）和工业污水处理厂（ISTPs）。本研究通过高通量 qPCR 和高通量 Illumina 测序，对鄱阳湖附近的两个市政污水处理厂和一个工业污水处理厂的 ARGs、移动遗传元件（MGEs）和细菌群落进行了全面调查。结果表明，ISTP 和两个 MSTPs 之间的 ARGs、MGEs 和细菌的特征存在差异，这可能是由于进水水质的差异造成的，例如 ISTP 进水的 Pb 特征。两个 MSTPs 的水力停留时间（HRT）比 ISTP 长，这也可能是造成差异的原因之一。因此，CASS 工艺中较长的水力停留时间似乎可以更广泛地去除具有相似处理工艺的 ISTPs 中的 ARGs 和细菌。通过全面了解选定的 MSTPs 和 ISTP 的 ARGs、MGEs 和细菌群落的特征，本研究为控制不同类型 STPs 中 ARGs 和 ARB 的传播和扩散提供了科学依据。
