Kraas E, Löhde E, Abri O, Schlicker H, Matzku S, Kalthoff H, Schmiegel W, Arndt R
Department of Surgery, Moabit Hospital, Free University, Berlin, FRG.
Int J Cancer Suppl. 1988;3:77-82.
We describe a model for the evaluation of anti-tumour antibody specificity, using a human carcinoma-bearing colon segment. After resection of the human colon tumour, the supplying artery was cannulated and perfused with fresh frozen plasma and heparin. Continuous control of pressure, flow, temperature, pH and various metabolic parameters were performed after administration of 131I-labelled anti-CEA antibody. Highly differentiated adenocarcinomas of the colon showed a much higher antibody uptake than undifferentiated tumours. Between 3 and 7% of the injected antibody was found in the tumour tissue. Autoradiography showed non-homogeneous binding in the tumour tissue. The non-specific antibody perfusion showed no tumour binding. We conclude that the ex vivo perfusion of resected colon carcinomas can be used to measure the kinetics of binding and clearance of MAbs in tumour tissue by direct scintigraphy. The cellular biodistribution of the antibody can be documented by means of autoradiography.
我们描述了一种使用携带人类癌组织的结肠段评估抗肿瘤抗体特异性的模型。切除人类结肠肿瘤后，将供应动脉插管并用新鲜冷冻血浆和肝素进行灌注。在给予¹³¹I标记的抗癌胚抗原（CEA）抗体后，持续监测压力、流量、温度、pH值和各种代谢参数。高分化结肠癌的抗体摄取量比未分化肿瘤高得多。在肿瘤组织中发现注入抗体的3%至7%。放射自显影显示肿瘤组织中的结合不均匀。非特异性抗体灌注未显示肿瘤结合。我们得出结论，切除的结肠癌的体外灌注可用于通过直接闪烁显像测量单克隆抗体（MAbs）在肿瘤组织中的结合和清除动力学。抗体的细胞生物分布可通过放射自显影记录。
