Chailurkit La-Or, Paiyabhroma Nitchawat, Sritara Piyamit, Vathesatogkit Prin, Yamwong Sukit, Thonmung Nisakron, Ongphiphadhanakul Boonsong
Department of Medicine, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Rama 6th Road, Bangkok 10400, Thailand.
Office of Research, Academic Affairs and Innovations, Faculty of Medicine, Ramathibodi Hospital, Mahidol University, Bangkok 10400, Thailand.
Metabolites. 2020 Feb 20;10(2):76. doi: 10.3390/metabo10020076.
Branched-chain amino acids (BCAAs) and lysophosphatidylcholines (LPCs) have been reported to be associated with diabetes. The purpose of the present study was to investigate the relative contributions of BCAAs and LPCs to the progression of prediabetes to diabetes using a targeted metabolomic approach. This study was part of a health survey of employees of the Electricity Generating Authority of Thailand ( = 79; nine females and 70 males). A targeted metabolomics analysis was performed using an AbsoluteIDQ p180 kit, flow injection analysis, and liquid chromatography-tandem mass spectrometry. The highest variable importance in projection (VIP) scores for the progression to diabetes of the amino acids and phospholipids were associated with isoleucine and LPC acyl C28:1, respectively. Using logistic regression analysis, we found that high baseline isoleucine concentration was associated with a higher incidence of diabetes, while high LPC acyl 28:1 was associated with a lower incidence. Isoleucine and LPC acyl 28:1 were independently associated with incident diabetes in a model that also included conventional risk factors for diabetes (baseline fasting plasma glucose (FPG), age, sex, and body mass index (BMI)). In addition, isoleucine and LPC acyl 28:1 were independently associated with serum HbA1c 5 years later in a robust regression model that also included baseline FPG, age, sex, and BMI. Isoleucine, LPC acyl 28:1, age, and FPG were significantly associated with HbA1c at this time. In conclusion, these results provide evidence that isoleucine and LPC acyl C28:1 have respective positive and negative independent associations with incident diabetes.
据报道，支链氨基酸（BCAAs）和溶血磷脂酰胆碱（LPCs）与糖尿病有关。本研究的目的是使用靶向代谢组学方法研究BCAAs和LPCs在糖尿病前期进展为糖尿病过程中的相对作用。本研究是泰国发电管理局员工健康调查的一部分（n = 79；9名女性和70名男性）。使用AbsoluteIDQ p180试剂盒、流动注射分析和液相色谱 - 串联质谱法进行靶向代谢组学分析。氨基酸和磷脂进展为糖尿病的最高投影变量重要性（VIP）分数分别与异亮氨酸和LPC酰基C28:1相关。使用逻辑回归分析，我们发现高基线异亮氨酸浓度与糖尿病发病率较高相关，而高LPC酰基28:1与较低发病率相关。在一个还包括糖尿病传统危险因素（基线空腹血糖（FPG）、年龄、性别和体重指数（BMI））的模型中，异亮氨酸和LPC酰基28:1与糖尿病发病独立相关。此外，在一个同样包括基线FPG、年龄、性别和BMI的稳健回归模型中，异亮氨酸和LPC酰基28:1与5年后的血清糖化血红蛋白（HbA1c）独立相关。此时，异亮氨酸、LPC酰基28:1、年龄和FPG与HbA1c显著相关。总之，这些结果提供了证据，表明异亮氨酸和LPC酰基C28:1分别与糖尿病发病存在正向和负向独立关联。
