Department of Molecular Biology, Institute of Parasitology and Biomedicine "López-Neyra" (IPBLN-CSIC), PTS, 18016 Granada, Spain.
Department of Cell Biology and Immunology, Institute of Parasitology and Biomedicine "López-Neyra" (IPBLN-CSIC), PTS, 18016 Granada, Spain.
J Cell Sci. 2020 Apr 7;133(7):jcs239509. doi: 10.1242/jcs.239509.
In eukaryotes, a large amount of histones need to be synthesized during the S phase of the cell cycle to package newly synthesized DNA into chromatin. The transcription and 3' end processing of histone pre-mRNAs are controlled by the histone locus body (HLB), which is assembled on the shared promoter for and Here, we identified the Prp40 pre-mRNA processing factor (dPrp40, annotated as CG3542) as a novel HLB component. We showed that dPrp40 is essential for development, with functionally conserved activity in vertebrates and invertebrates. We observed that dPrp40 is fundamental in endocycling cells, highlighting a role for this factor in mediating replication efficiency The depletion of dPrp40 from fly cells inhibited the transcription, but not the 3' end processing, of histone mRNA in a - and -promoter-dependent manner. Our results establish that dPrp40 is an essential protein for development that can localize to the HLB and might participate in histone mRNA biosynthesis.
在真核生物中，细胞周期 S 期需要大量合成组蛋白，将新合成的 DNA 包装成染色质。组蛋白前体 mRNA 的转录和 3' 端加工受组蛋白基因座体 (HLB) 控制，该结构组装在 和 的共享启动子上。在这里，我们鉴定了 Prp40 前体 mRNA 加工因子 (dPrp40，注释为 CG3542) 为一个新的 HLB 成分。我们表明 dPrp40 对 的发育是必需的，在脊椎动物和无脊椎动物中具有功能保守的活性。我们观察到 dPrp40 在细胞内循环中是基本的，这突出了该因子在介导复制效率方面的作用。从果蝇细胞中耗尽 dPrp40 以 - 和 - 启动子依赖的方式抑制了组蛋白 mRNA 的转录，但不抑制 3' 端加工。我们的结果表明，dPrp40 是 发育所必需的蛋白，它可以定位于 HLB，并可能参与组蛋白 mRNA 的生物合成。
