初榨椰子油与富含生育三烯酚的组分联合使用可预防骨质疏松大鼠模型的骨质流失。

Combined virgin coconut oil and tocotrienol-rich fraction protects against bone loss in osteoporotic rat model.

作者信息

Malik Mohd Maaruf Abdul, Othman Faizah, Hussan Farida, Shuid Ahmad Nazrun, Saad Qodriyah Mohd

机构信息

Department of Anatomy, Faculty of Medicine, Universiti Kebangsaan Malaysia Medical Centre, Jalan Yaacob Latif, 56000 Cheras, Kuala Lumpur, Malaysia.

Centre of Preclinical Science Studies, Faculty of Dentistry, Universiti Teknologi MARA, Sungai Buloh Campus, Jalan Hospital, 47000 Sungai Buloh, Selangor, Malaysia.

出版信息

Vet World. 2019 Dec;12(12):2052-2060. doi: 10.14202/vetworld.2019.2052-2060. Epub 2019 Dec 25.

DOI:10.14202/vetworld.2019.2052-2060

PMID:32095059

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6989331/

Abstract

BACKGROUND AND AIM

Both virgin coconut oil (VCO) and tocotrienol-rich fraction (TRF) are rich in antioxidants and may protect the bone against bone loss induced by ovariectomy and high-fat diet. The study aimed to determine the protective effects of combined therapy of VCO and TRF on osteoporosis in ovariectomized (OVX) rat fed with high-fat diet.

MATERIALS AND METHODS

Thirty-six female Sprague-Dawley rats were divided into six groups: Sham-operated (SHAM), OVX control, OVX and given Premarin at 64.5 µg/kg (OVX+E2), OVX and given VCO at 4.29 ml/kg (OVX+V), OVX and given TRF at 30 mg/kg (OVX+T), and OVX and given a combination of VCO at 4.29 ml/kg and TRF at 30 mg/kg (OVX+VT). Following 24 weeks of treatments, blood and femora samples were taken for analyses.

RESULTS

There were no significant differences in serum osteocalcin levels between the groups (p>0.05), while serum C-terminal telopeptide of Type I collagen levels of the OVX+VT group were significantly lower than the other groups (p<0.05). The dynamic bone histomorphometry analysis of the femur showed that the double-labeled surface/bone surface (dLS/BS), mineral apposition rate, and bone formation rate/BS of the OVX+E2, OVX+T, and OVX+VT groups were significantly higher than the rest of the groups (p<0.05).

CONCLUSION

A combination of VCO and TRF has the potential as a therapeutic agent to restore bone loss induced by ovariectomy and high-fat diet.

摘要

背景与目的

初榨椰子油（VCO）和富含生育三烯酚的组分（TRF）均富含抗氧化剂，可能保护骨骼免受卵巢切除和高脂饮食引起的骨质流失。本研究旨在确定VCO与TRF联合治疗对高脂饮食喂养的去卵巢（OVX）大鼠骨质疏松症的保护作用。

材料与方法

36只雌性Sprague-Dawley大鼠分为六组：假手术组（SHAM）、OVX对照组、OVX并给予64.5μg/kg的倍美力组（OVX+E2）、OVX并给予4.29ml/kg的VCO组（OVX+V）、OVX并给予30mg/kg的TRF组（OVX+T）以及OVX并给予4.29ml/kg的VCO与30mg/kg的TRF联合组（OVX+VT）。经过24周的治疗后，采集血液和股骨样本进行分析。

结果

各组间血清骨钙素水平无显著差异（p>0.05），而OVX+VT组的血清I型胶原C端肽水平显著低于其他组（p<0.05）。股骨的动态骨组织形态计量学分析表明，OVX+E2组、OVX+T组和OVX+VT组的双标记表面/骨表面（dLS/BS）、矿物质沉积率以及骨形成率/BS显著高于其他组（p<0.05）。