长链非编码 RNA CHRF 通过 EMT 促进胃癌细胞侵袭和迁移。
LncRNA CHRF promotes cell invasion and migration via EMT in gastric cancer.
机构信息
Department of Internal Medicine-Oncology, Huangshi Central Hospital, Pu'ai District, Affiliated Hospital of Hubei Polytechnic University, Edong Healthcare Group, Huangshi, China.
出版信息
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1168-1176. doi: 10.26355/eurrev_202002_20168.
OBJECTIVE
Long non-coding RNAs (lncRNAs) have been verified to involve in the development and progression of gastric cancer (GC). However, the expression of lncRNA CHRF level in GC has not been mentioned before. Here, we focused on the function of lncRNA CHRF played in GC.
PATIENTS AND METHODS
A total of 103 GC tissues and paired para-tumor tissues from GC patients were collected. The quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was applied to measure the lncRNA CHRF level in these samples and GC cell lines. The Wound-healing experiment, transwell assay, and Matrigel assay were employed to study the migration and invasion abilities of GC cells. The underlying molecular of lncRNA CHRF was measured using Western-blot.
RESULTS
LncRNA CHRF expression was significantly higher in 103 GC tissue samples compared with the adjacent para-tumor samples. In GC cells, lncRNA CHRF showed increased expression levels than the human fetal gastric epithelial cells (GES-1). Inhibition of lncRNA CHRF reduced the invasion and migration of MKN-7 cells while the over-expression of lncRNA CHRF promoted HGC-27 cells metastasis. Furthermore, we found that lncRNA CHRF could promote the progression of epithelial-mesenchymal transition (EMT) to promote the GC cell metastasis.
CONCLUSIONS
Our current study demonstrated that lncRNA CHRF functioned as an oncogene in GC and promoted cell invasion and migration via EMT. This might furnish a potential target for the GC biological diagnosis and therapy.
目的
长链非编码 RNA（lncRNA）已被证实参与胃癌（GC）的发生和发展。然而，lncRNA CHRF 在 GC 中的表达尚未提及。在这里，我们专注于 lncRNA CHRF 在 GC 中发挥的功能。
患者与方法
共收集了 103 例 GC 组织和配对的 GC 患者癌旁组织。采用定量实时聚合酶链反应（qRT-PCR）检测这些样本和 GC 细胞系中 lncRNA CHRF 的水平。采用划痕实验、Transwell 实验和 Matrigel 实验研究 GC 细胞的迁移和侵袭能力。采用 Western blot 检测 lncRNA CHRF 的潜在分子机制。
结果
与相邻癌旁组织相比，103 例 GC 组织样本中 lncRNA CHRF 的表达明显升高。在 GC 细胞中，lncRNA CHRF 的表达水平高于人胎儿胃上皮细胞（GES-1）。lncRNA CHRF 的抑制降低了 MKN-7 细胞的侵袭和迁移能力，而过表达 lncRNA CHRF 促进了 HGC-27 细胞的转移。此外，我们发现 lncRNA CHRF 可以促进上皮-间充质转化（EMT）的进展，从而促进 GC 细胞的转移。
结论
本研究表明，lncRNA CHRF 在 GC 中作为癌基因发挥作用，并通过 EMT 促进细胞侵袭和迁移。这可能为 GC 的生物诊断和治疗提供潜在的靶点。
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