Shi Y-B, Liu S-L, Mou X-R, Liao J, Che J-P, Fei X-Q, Wang A-R
Department of Cardiothoracic Surgery, Yantai Mountain Hospital, Yantai, China.
Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1243-1249. doi: 10.26355/eurrev_202002_20177.
Recent studies have proved that long non-coding RNAs (lncRNAs) play important roles in many diseases, especially malignancies. The aim of this study was to investigate the exact role of lncRNA HOXA-AS2 (Hoxa cluster antisense RNA 2) in the development of non-small cell lung cancer (NSCLC).
Quantitative Real Time-Polymerase Chain Reaction (qRT-PCR) was utilized to detect HOXA-AS2 expression in NSCLC patients. The Wound healing assay and transwell assay were conducted to explore the function of HOXA-AS2 on NSCLC metastasis. Furthermore, the mechanism assays were used to explore the interaction between HOXA-AS2 and microRNA-145-3p (miR-145-3p).
HOXA-AS2 expression level in NSCLC tissues was significantly higher than adjacent tissues. HOXA-AS2 expression was negatively correlated with disease-free survival of NSCLC patients. Moreover, the functional assays showed that the migration and invasion of NSCLC cells were significantly inhibited after HOXA-AS2 in vitro silence. Furthermore, the luciferase reporter gene assay also revealed that miR-145-3p was a direct target of HOXA-AS2 in NSCLC.
Our results indicated that HOXA-AS2 could enhance the migration and invasion abilities of NSCLC by targeting miR-145-3p. Furthermore, these findings suggested that HOXA-AS2 might be a potential therapeutic target for NSCLC.
近期研究已证明长链非编码RNA（lncRNAs）在许多疾病尤其是恶性肿瘤中发挥重要作用。本研究旨在探讨长链非编码RNA HOXA-AS2（Hoxa簇反义RNA 2）在非小细胞肺癌（NSCLC）发生发展中的具体作用。
采用定量实时聚合酶链反应（qRT-PCR）检测NSCLC患者中HOXA-AS2的表达。进行伤口愈合试验和Transwell试验以探究HOXA-AS2对NSCLC转移的作用。此外，运用机制分析来探究HOXA-AS2与微小RNA-145-3p（miR-145-3p）之间的相互作用。
NSCLC组织中HOXA-AS2表达水平显著高于癌旁组织。HOXA-AS2表达与NSCLC患者无病生存期呈负相关。此外，功能试验表明HOXA-AS2体外沉默后NSCLC细胞的迁移和侵袭能力显著受到抑制。而且，荧光素酶报告基因试验还显示miR-145-3p是NSCLC中HOXA-AS2的直接靶点。
我们的结果表明HOXA-AS2可通过靶向miR-145-3p增强NSCLC的迁移和侵袭能力。此外，这些发现提示HOXA-AS2可能是NSCLC的一个潜在治疗靶点。
