Hsa_circ_0011946 通过诱导 EMT 过程促进肝癌的迁移和侵袭。

Hsa_circ_0011946 promotes the migration and invasion of hepatocellular carcinoma by inducing EMT process.

机构信息

Department of Radiology, The First Affiliated Hospital of China Medical University, Shenyang, China.

出版信息

Eur Rev Med Pharmacol Sci. 2020 Feb;24(3):1108-1115. doi: 10.26355/eurrev_202002_20161.

DOI:10.26355/eurrev_202002_20161

PMID:32096173

Abstract

OBJECTIVE

Recently, the vital role of circular RNAs is discovered in many diseases including tumor progression and metastasis. Hepatocellular carcinoma (HCC) is one of the most ordinary malignant tumors. The purpose of our study is to detect the potential function of hsa_circ_0011946 in HCC to offer new biomarkers and targets.

PATIENTS AND METHODS

The level of hsa_circ_0011946 in HCC tissues and cell lines was monitored by Real Time Quantitative Polymerase Chain Reaction (RT-qPCR). Pearson's Chi-square test was used to determine the association between hsa_circ_0011946 expression and several clinicopathological factors. Then, hsa_circ_0011946 was knocked down in HCC cells to uncover its function in metastasis of HCC. Cell migrated and invaded ability was measured through transwell assay, Matrigel assay and wound healing assay. Western blot assay was performed to analyze the effect of hsa_circ_0011946 on the epithelial-to-mesenchymal transition (EMT) process.

RESULTS

In this research, the expression level of hsa_circ_0011946 was significantly increased in HCC tissues compared to that in adjacent samples. The expression of hsa_circ_0011946 was also increased in HCC cell lines. The hsa_circ_0011946 expression was associated with lymphatic metastasis in HCC patients. Knockdown of hsa_circ_0011946 led to the inhibition of cell migration and invasion in HCC. In addition, results of further experiments revealed that the EMT-related proteins were regulated via the knockdown of hsa_circ_0011946 in HCC.

CONCLUSIONS

The hsa_circ_0011946 could enhance cell migration and invasion of HCC by inducing the EMT process.

摘要

目的

最近，环状 RNA 在包括肿瘤进展和转移在内的许多疾病中发挥着重要作用。肝细胞癌（HCC）是最常见的恶性肿瘤之一。我们的研究旨在检测 hsa_circ_0011946 在 HCC 中的潜在功能，为其提供新的生物标志物和靶点。

方法

通过实时定量聚合酶链反应（RT-qPCR）监测 HCC 组织和细胞系中 hsa_circ_0011946 的水平。Pearson's Chi-square 检验用于确定 hsa_circ_0011946 表达与几种临床病理因素之间的关联。然后，在 HCC 细胞中敲低 hsa_circ_0011946，以揭示其在 HCC 转移中的作用。通过 Transwell 测定、Matrigel 测定和划痕愈合测定来测量细胞迁移和侵袭能力。Western blot 测定用于分析 hsa_circ_0011946 对上皮间质转化（EMT）过程的影响。

结果

在这项研究中，与相邻样本相比，HCC 组织中 hsa_circ_0011946 的表达水平显著增加。HCC 细胞系中也增加了 hsa_circ_0011946 的表达。hsa_circ_0011946 的表达与 HCC 患者的淋巴转移有关。敲低 hsa_circ_0011946 导致 HCC 细胞迁移和侵袭的抑制。此外，进一步实验的结果表明，通过敲低 hsa_circ_0011946 可调节 HCC 中的 EMT 相关蛋白。