Separation of triglyceride groups by reversed-phase thin-layer chromatography on silanized Kieselguhr.

The resolution of triglyceride mixtures on a Kieselguhr G layer silanized with dimethyldichlorosilane and the mobile phase acetone-acetonitrile-water under different chromatographic conditions has been investigated. The influence of the water content on the separation has been studied. Each triglyceride mixture needs a mobile phase with a definite polarity, which depends on the partition numbers of the triglyceride components. Since a linear correlation between the partition number and the water content was observed, prediction of the mobile phase composition for any triglyceride mixture is possible, provided the partition numbers of the components are known. The chromatographic conditions found can be applied for resolution of triglyceride groups with partition numbers ranging from 30 to 52, in different triglyceride mixtures.