Abdulateef Darya S, Salih Jamal M
Physiology Department, College of Medicine, University of Sulaimani, Sulaymaniyah, Kurdistan Region, Iraq.
Diabetes Physician, Diabetes and Endocrine Centre, Sulaymaniyah, Kurdistan Region, Iraq.
Diabetes Metab Syndr Obes. 2020 Feb 3;13:227-236. doi: 10.2147/DMSO.S240785. eCollection 2020.
The C-peptide level from blood and urine samples represents endogenous insulin secretion and has clinical implications for individuals with and without diabetes. However, the study results are inconsistent and the available methods can only measure short-term C-peptide levels, which can be substantially affected by concurrent glycaemia. In this study, we evaluated whether C-peptide can be detected from hair? Hair C-peptide if detected could potentially represent long-term, insulin secretion. And to know whether there is any correlation between hair C-peptide with certain biochemical and demographic parameters.
In a prospective observational study on 120 normal healthy individuals, hair samples were taken from the scalp and C-peptide was extracted. The hair C-peptide levels were measured in pg/mg of hair using electrochemiluminescence immunoassay (ECLISA) after methanol extraction and were compared among different age and body-type groups. Serum C-peptide, fasting plasma glucose (FPG) and HbA1c were assessed and their levels were correlated with anthropometric parameters.
A detectable range of C-peptide was found in the hair samples of all the subjects with a median of 63.59 pg/mg hair and it was positively correlated with FPG but no significant correlation was found between hair C-peptide and serum C-peptide levels. The hair C-peptide level, along with FPG and HbA1c, was significantly different according to age.
These results conclude that C-peptide can be detected from and measured in the scalp hair of humans and it is positively correlated with FPG, and it is significantly different according to age. This technique, if validated, may verify its usefulness in future studies of both healthy and diseased subjects.
血液和尿液样本中的C肽水平代表内源性胰岛素分泌，对糖尿病患者和非糖尿病患者均具有临床意义。然而，研究结果并不一致，现有的方法只能测量短期C肽水平，而这可能会受到同时存在的血糖水平的显著影响。在本研究中，我们评估了是否可以从头发中检测到C肽？如果检测到头发C肽，则可能代表长期的胰岛素分泌。并了解头发C肽与某些生化和人口统计学参数之间是否存在相关性。
在一项对120名正常健康个体的前瞻性观察研究中，从头皮采集头发样本并提取C肽。使用电化学发光免疫分析法（ECLISA）在甲醇提取后以皮克/毫克头发为单位测量头发C肽水平，并在不同年龄和体型组之间进行比较。评估血清C肽、空腹血糖（FPG）和糖化血红蛋白（HbA1c），并将它们的水平与人体测量参数相关联。
在所有受试者的头发样本中均发现了可检测范围的C肽，中位数为63.59皮克/毫克头发，并且它与FPG呈正相关，但头发C肽与血清C肽水平之间未发现显著相关性。根据年龄，头发C肽水平以及FPG和HbA1c存在显著差异。
这些结果表明，可以从人类头皮头发中检测和测量C肽，它与FPG呈正相关，并且根据年龄存在显著差异。如果该技术得到验证，可能会在未来对健康和患病受试者的研究中证实其有用性。
