Mousavi Nasab Seyed Dawood, Ahmadi Vasmehjani Abbas, Kaghazian Hooman, Mardani Rajab, Zali Fatemeh, Ahmadi Nayebali, Norouzinia Mohsen, Akbari Zahra
Department of Research and Development, Production and Research Complex, Pasteur Institute of Iran, Tehran, Iran.
Department of Microbiology and Immunology, Jahrom University of Medical Sciences, Jahrom, Iran.
Gastroenterol Hepatol Bed Bench. 2019;12(Suppl1):S156-S162.
The present study was designed to evaluate the correlation of interleukin 28B (IL28B, IFNL3) rs12979860 mRNA levels, viral load, and liver function among hepatitis C virus (HCV) patients genotype 1a.
HCV is considered essentially hepatotropic and is a major health problem around the world.
This study included 100 HCV-infected patients with HCV genotype1a (G1a) and rs12979860 CC genotype. These patients were divided into two groups according to HCV treatment. Aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), and HCV Load were measured and recorded for each patient. IL28B mRNA levels were determined using real-time polymerase chain reaction assay, and their correlation with clinical data were analyzed. STRING was applied to construct a network and identify interactions between IL28B () and its significant neighbor proteins.
The results revealed a significant relationship between the ALT as well as ALP levels with IL28B rs12979860 mRNA expression level in men, and also with age >50 years. In the treated group, AST level and HCV load had a significant relationship with IL28B mRNA expression level. The results showed that the level of ALP and AST decreased significantly with increased IL28B mRNA expression level in the treated and untreated group, respectively. STRING database showed that IL28B (IFNL3) interacted with ten important neighbor proteins with some of these proteins being involved in signal transduction pathway activating antiviral response.
This study indicated that rs12979860CC genotype could predict IL28B mRNA expression level in HCV-infected patients with G1a. Furthermore, IL28B mRNA expression level may serve as a useful marker for the development of G1a HCV-associated outcomes.
本研究旨在评估丙型肝炎病毒（HCV）1a基因型患者中白细胞介素28B（IL28B，IFNL3）rs12979860 mRNA水平、病毒载量和肝功能之间的相关性。
HCV本质上被认为是嗜肝性的，是全球主要的健康问题。
本研究纳入了100例HCV感染的1a基因型（G1a）且rs12979860 CC基因型患者。根据HCV治疗情况将这些患者分为两组。测量并记录每位患者的天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）、碱性磷酸酶（ALP）和HCV载量。使用实时聚合酶链反应测定法测定IL28B mRNA水平，并分析其与临床数据的相关性。应用STRING构建网络并识别IL28B及其重要邻近蛋白之间的相互作用。
结果显示，男性患者的ALT以及ALP水平与IL28B rs12979860 mRNA表达水平之间存在显著关系，且与年龄>50岁也有关系。在治疗组中，AST水平和HCV载量与IL28B mRNA表达水平存在显著关系。结果表明，在治疗组和未治疗组中，随着IL28B mRNA表达水平升高，ALP和AST水平分别显著下降。STRING数据库显示，IL28B（IFNL3）与十种重要的邻近蛋白相互作用，其中一些蛋白参与激活抗病毒反应的信号转导途径。
本研究表明，rs12979860 CC基因型可预测G1a型HCV感染患者的IL28B mRNA表达水平。此外，IL28B mRNA表达水平可能是G1a型HCV相关结局发生的有用标志物。
