Normand Valérie, Boulbria Gwenaël, Brissonnier Mathieu, Bachy Véronique, Moalic Pierre-Yves, Berton Pauline, Bouchet Franck, Lebret Arnaud
Porc.Spective Swine Vet Practice, ZA de Gohélève, rue Joseph et Etienne Montgolfier, 56920 Noyal Pontivy, France.
Orbio Finalab Veterinary Laboratory Group, 12 rue du 35ème regiment d'aviation, 69500 Bron, France.
Porcine Health Manag. 2020 Feb 20;6:3. doi: 10.1186/s40813-019-0143-8. eCollection 2020.
() is an haemotropic Mycoplasma that adheres and invades erythrocytes and is responsible for infectious anaemia of pigs. Infections with have been reported worldwide. Clinical signs after infection can be significant particularly for the breeding herd in the period around farrowing but consequences are highly variable with some infected pigs never exhibiting clinical disease. The study aimed to determine the clinical relevance of Giemsa-stained blood smear for the diagnosis of compared with qPCR results. In our study, the comparison of qPCR results with microscopic investigation of Giemsa-stained blood smears revealed a lower sensitivity of the microscopic method: only 33 out of 102 qPCR positive blood samples were microscopically positive ( visualised). No relationship between mean qPCR loads and microscopic observation was observed. Although more costly, qPCR is probably the best diagnostic tool available today for diagnosis.
（某病原体名称）是一种嗜血性支原体，它黏附并侵入红细胞，是猪传染性贫血的病原体。世界各地均有该病原体感染的报道。感染该病原体后的临床症状可能较为显著，尤其是在分娩前后的繁殖猪群中，但后果差异很大，一些感染猪从未表现出临床疾病。该研究旨在确定与qPCR结果相比，吉姆萨染色血涂片对该病原体诊断的临床相关性。在我们的研究中，将qPCR结果与吉姆萨染色血涂片的显微镜检查结果进行比较，发现显微镜检查方法的灵敏度较低：102份qPCR阳性血样中只有33份在显微镜下呈阳性（可见）。未观察到平均qPCR载量与显微镜观察之间的关系。尽管成本更高，但qPCR可能是目前可用于该病原体诊断的最佳诊断工具。
