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Dataset on optimization of lignin peroxidase production by sp. under submerged fermentation using one factor at a time approach.

作者信息

Nayana P, Aiswarya C, Aparna C K, Nambisan Padma

机构信息

Plant Biotechnology Laboratory, Department of Biotechnology, Cochin University of Science and Technology, Cochin 682022, Kerala, India.

Indian Institute of Science Education and Research, Tirupati, Andhra Pradesh 517507, India.

出版信息

Data Brief. 2020 Feb 6;29:105244. doi: 10.1016/j.dib.2020.105244. eCollection 2020 Apr.

DOI:10.1016/j.dib.2020.105244
PMID:32099876
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7026465/
Abstract

One factor at a time approach was employed for the optimization of media components, physical factors and additional additives for lignin peroxidase (LiP) production under submerged fermentation by the endophytic fungus sp. The optimized conditions derived were: carbon source (dextrose 10 g/L), nitrogen source (ammonium tartarate 0.027 g/L), veratryl alcohol (1 mM), MnSO (0.5 g/L), inoculum concentration (3 mycelial plugs), pH (5), temperature (30 °C) and Tween 80 (1.5%). A 40 fold increase of enzyme activity was observed after the single parameter optimization. The lignin peroxidase (LiP) production to the level of 345.26 ± 0.52 IU/mL indicates that the fungus has the commercial potential for LiP.

摘要
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/3f65a9642fe4/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/977fb359019c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/c615440c325b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/ef8417c0c5ac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/ca13d525be5b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/3f65a9642fe4/gr5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/977fb359019c/gr1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/c615440c325b/gr2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/ef8417c0c5ac/gr3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/ca13d525be5b/gr4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/484b/7026465/3f65a9642fe4/gr5.jpg

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本文引用的文献

1
Lignin-degrading enzyme from Phanerochaete chrysosporium: Purification, characterization, and catalytic properties of a unique H(2)O(2)-requiring oxygenase.白腐真菌木质素降解酶:一种独特的需要 H(2)O(2)的氧化酶的纯化、表征和催化特性。
Proc Natl Acad Sci U S A. 1984 Apr;81(8):2280-4. doi: 10.1073/pnas.81.8.2280.
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Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.