IIIUC - Institute for Interdisciplinary Research, University of Coimbra, Coimbra, Portugal.
CNC - Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra, Portugal.
Microbiology (Reading). 2020 May;166(5):474-483. doi: 10.1099/mic.0.000898.
is so far the most thermophilic among mycobacteria as it grows optimally at 50 °C and up to 65 °C in a glycerol-based medium, as verified in this study. Since this and other nontuberculous mycobacteria (NTM) thrive in diverse natural and artificial environments, from where they may access and infect humans, we deemed essential to probe resistance to heat, a strategy routinely used to control microbial growth in water-supply systems, as well as in the food and drink industries. In addition to possibly being a threat in its own right in rare occasions, is also a good surrogate for studying other NTM species more often associated with opportunistic infection, namely and as well as their strictly pathogenic counterparts and . In this regard, this thermophilic species is likely to be useful as a source of stable proteins that may provide more detailed structures of potential drug targets. Here, we investigate growth at near-pasteurization temperatures and at different pHs and also characterize its thermostable glucosyl-3-phosphoglycerate synthase (GpgS), an enzyme considered essential for growth and associated with both nitrogen starvation and thermal stress in different NTM species.
到目前为止，它是分枝杆菌中最耐热的，因为在本研究中，它在基于甘油的培养基中最适生长温度为 50°C 至 65°C。由于这种和其他非结核分枝杆菌（NTM）在各种自然和人工环境中大量存在，它们可能会从这些环境中进入并感染人类，因此我们认为有必要研究耐热性，这是一种在供水系统以及食品和饮料行业中常规用于控制微生物生长的策略。除了在罕见情况下可能本身构成威胁之外，它还是研究其他通常与机会性感染相关的 NTM 物种的良好替代品，即 和 以及它们严格的致病性对应物 和 。在这方面，这种嗜热物种可能是作为稳定蛋白的来源有用，这些蛋白可能为潜在药物靶标的提供更详细的结构。在这里，我们研究了 在接近巴氏消毒温度和不同 pH 值下的生长情况，还对其热稳定的葡糖基-3-磷酸甘油酸合酶（GpgS）进行了表征，这种酶被认为是分枝杆菌生长所必需的，与不同 NTM 物种中的氮饥饿和热应激有关。
