Departamento de Produção e Melhoramento Vegetal, Faculdade de Ciências Agronômicas, Universidade Estadual Paulista, Botucatu, São Paulo, Brazil.
Instituto de Ciências Agrárias, Universidade Federal de Uberlândia, Uberlândia, Minas Gerais, Brazil.
PLoS One. 2020 Feb 26;15(2):e0229215. doi: 10.1371/journal.pone.0229215. eCollection 2020.
We hypothesize that by simulating the natural priming in seeds of a species that forms transient seed banks it is possible to clarify molecular aspects of germination that lead to the recruitment of seedlings when the next rainy season begins. We used seeds of Solanum lycocarpum as a biological model. Our findings support the idea that the increment of seed germination kinetics when the rainy season returns is mainly based on the metabolism and embryonic growth, and that the hydropriming, at the end of seed dispersion, increases the germination window time of these seeds by mainly increasing the degradation of galactomannan of the cell wall. This can improve the energy supply (based on carbon metabolism) for seedling growth in post-germination, which improves the seedling's survival chances. From these findings, we promote a hypothetical model about how the priming at the end of the rainy season acts on mRNA synthesis in the germination of seeds from transient banks and the consequence of this priming at the beginning of the following rainy season. This model predicts that besides the Gibberellin and Abscisic Acid balance (content and sensitivity), Auxin would be a key component for the seed-seedling transition in Neotropical areas. Seed collection was performed under authorization number SISGEN AB0EB45.
我们假设通过模拟形成暂态种子库的物种种子中的自然引发作用，有可能阐明导致下一个雨季开始时幼苗出现的萌发的分子方面。我们使用 Solanum lycocarpum 的种子作为生物模型。我们的研究结果支持这样的观点，即雨季回归时种子萌发动力学的增加主要基于代谢和胚胎生长，并且在种子分散结束时的水引发主要通过增加细胞壁半乳甘露聚糖的降解来增加这些种子的萌发窗口时间。这可以改善萌发后幼苗生长的能量供应（基于碳代谢），从而提高幼苗的生存机会。根据这些发现，我们提出了一个关于雨季结束时的引发作用如何在暂态种子库的种子萌发中影响 mRNA 合成以及这种引发作用在下一个雨季开始时的后果的假设模型。该模型预测，除了赤霉素和脱落酸的平衡（含量和敏感性）之外，生长素将是新热带地区种子-幼苗过渡的关键组成部分。种子采集得到 SISGEN AB0EB45 授权号的授权。
