REQUIMTE/LAQV, Instituto Superior de Engenharia do Porto, Instituto Politécnico do Porto, Porto, Portugal.
Instituto de Investigação e Inovação em Saúde, i3S, Universidade do Porto, Porto, Portugal.
PLoS One. 2020 Feb 26;15(2):e0229659. doi: 10.1371/journal.pone.0229659. eCollection 2020.
The cultivation of genetically modified organisms (GMO) continues to expand worldwide. Still, many consumers express concerns about the use of GMO in food or feed, and many countries have legislated on labelling systems to indicate the presence of GMO in commercial products. To deal with the increased number of GMO events and to address related regulations, alternative detection methods for GMO inspection are required. In this work, a genosensor based on Surface Plasmon Resonance under continuous flow was developed for the detection and quantification of a genetically modified soybean (event GTS 40-3-2). In a single chip, the simultaneous detection of the event-specific and the taxon-specific samples were achieved, whose detection limits were 20 pM and 16 pM, respectively. The reproducibility was 1.4%, which supports the use of the chip as a reliable and cost-effective alternative to other DNA-based techniques. The results indicate that the proposed method is a versatile tool for GMO quantification in food and feed samples.
转基因生物（GMO）的培养在全球范围内不断扩大。尽管如此，许多消费者对食品或饲料中使用 GMO 表示担忧，许多国家已经立法规定了标签系统，以表明商业产品中存在 GMO。为了应对越来越多的 GMO 事件并解决相关法规问题，需要替代的 GMO 检测方法。在这项工作中，开发了一种基于连续流动的表面等离子体共振的基因传感器，用于检测和定量一种转基因大豆（事件 GTS 40-3-2）。在单个芯片上，实现了事件特异性和分类群特异性样品的同时检测，其检测限分别为 20 pM 和 16 pM。重现性为 1.4%，这支持了该芯片作为其他基于 DNA 的技术的可靠且具有成本效益的替代方法的使用。结果表明，该方法是一种用于食品和饲料样品中 GMO 定量的多功能工具。
