Unidad de Investigación Genética, Posgrado en Ciencias Químico Biológicas, Facultad de Química, Universidad Autónoma de Querétaro, Centro Universitario, 76010 Querétaro, Mexico.
Centro de Investigación en Biología de la Reproducción del Instituto de Ciencias de la Salud, Universidad Autónoma del Estado de Hidalgo, 42082 Pachuca, Hidalgo, Mexico.
Medicina (Kaunas). 2020 Feb 23;56(2):90. doi: 10.3390/medicina56020090.
: Prostate cancer is the second most harmful disease in men worldwide and the number of cases is increasing. Therefore, new natural agents with anticancer potential should be examined and the response of existing therapeutic drugs must be enhanced. and are two species that have been widely used in traditional medicine, but their effectiveness on cancer cells and their interaction with antineoplastic drugs have not been studied. The aim of this study was to evaluate the anticancer activity of methanolic root extract () and methanolic root extract and their effect, combined with enzalutamide, on prostate cancer cells. The study was conducted on a human fibroblast cell line, and on androgen-dependent (LNCaP) and androgen-independent (PC-3) prostate cancer cell lines. The cell viability was evaluated using a Trypan Blue exclusion test for 48 h, and the migration by a wound-healing assay for 24, 48, and 72 h. The results indicate that and were not cytotoxic at concentrations less than 1000 μg/mL in the human fibroblasts. and significantly reduced the viability and migration of prostate cancer cells in all concentrations evaluated. The antiproliferative effect of the extracts was higher in cancer cells than in normal cells. The enzalutamide decreased the cell viability in all concentrations tested (10-50 µM). The combination of the extracts and enzalutamide produced a greater effect on the inhibition of the proliferation and migration of cancer cells than the extracts alone, but not of the enzalutamide alone. The results indicate that and have an inhibitory effect on the viability and migration of prostate cancer cells and do not interfere with the enzalutamide anticancer effect. The data suggest that extracts may be a potential source of molecules for cancer treatment.
前列腺癌是全球男性第二大危害性疾病，且其发病率呈上升趋势。因此，应该研究新的具有抗癌潜力的天然药物，增强现有治疗药物的疗效。 和 是两种在传统医学中广泛应用的物种，但它们对癌细胞的作用及其与抗肿瘤药物的相互作用尚未得到研究。本研究旨在评估 甲醇根提取物（）和 甲醇根提取物（）的抗癌活性，以及它们与恩杂鲁胺联合应用对前列腺癌细胞的影响。该研究在人成纤维细胞系和雄激素依赖性（LNCaP）和雄激素非依赖性（PC-3）前列腺癌细胞系上进行。用台盼蓝排斥试验在 48 h 内评估细胞活力，用划痕愈合试验在 24、48 和 72 h 内评估迁移。结果表明，在人成纤维细胞中，浓度小于 1000 μg/mL 时， 和 没有细胞毒性。 和 以所有评估浓度显著降低前列腺癌细胞的活力和迁移。与正常细胞相比，提取物对癌细胞的增殖抑制作用更高。在所有测试浓度（10-50 μM）下，恩杂鲁胺均降低细胞活力。与单独使用 提取物相比， 提取物与恩杂鲁胺联合使用对抑制癌细胞增殖和迁移的效果更大，但恩杂鲁胺单独使用则没有。结果表明， 和 对前列腺癌细胞的活力和迁移有抑制作用，且不干扰恩杂鲁胺的抗癌作用。数据表明， 提取物可能是癌症治疗潜在的分子来源。
