Department of Urology, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Research Center for Clinical Research, Jinshan Hospital, Fudan University, Shanghai, 201508, China.
Biochem Biophys Res Commun. 2020 Apr 30;525(2):418-424. doi: 10.1016/j.bbrc.2020.02.098. Epub 2020 Feb 23.
Collagen Triple Helix Repeat Containing 1 (CTHRC1) has been picked out as a cancer-related, secreted glycoprotein that possesses multifaceted functions such as wound repair, the formation of adipose tissue, hepatocytes fibrosis, and bone remodeling. This study aims to explore the biological function and the profound regulative mechanism of CTHRC1 in human prostate cancer (PCa). We found that CTHRC1 was upregulated in patients with PCa. The knockdown of CTHRC1 suppressed PCa cell proliferation, invasion, migration, and colony formation significantly. The expression of CTHRC1 was down-regulated and up-regulated by miR-30e-5p mimics and inhibitors, respectively, in PCa cells. The dual-luciferase reporter assay validated the binding of miR-30e-5p with CTHRC1 mRNA, indicating the regulation of CTHC1 by miR-30e-5p. In consequence, this study demonstrated that CTHRC1 acts as an oncogenic gene and targeting the miR-30e-5p-CTHRC1 axis may provide novel therapeutic treatment for PCa.
胶原蛋白三螺旋重复 1 型（CTHRC1）被认为是一种与癌症相关的分泌糖蛋白，具有多种功能，如伤口修复、脂肪组织形成、肝纤维化和骨重塑。本研究旨在探讨 CTHRC1 在人前列腺癌（PCa）中的生物学功能和深刻的调控机制。我们发现 CTHRC1 在 PCa 患者中上调。敲低 CTHRC1 显著抑制 PCa 细胞的增殖、侵袭、迁移和集落形成。miR-30e-5p 模拟物和抑制剂分别下调和上调了 PCa 细胞中 CTHRC1 的表达。双荧光素酶报告基因实验验证了 miR-30e-5p 与 CTHRC1 mRNA 的结合，表明 miR-30e-5p 对 CTHRC1 的调控作用。因此，本研究表明 CTHRC1 作为一种癌基因发挥作用，靶向 miR-30e-5p-CTHRC1 轴可能为 PCa 提供新的治疗方法。
