Department of Computer Engineering, Faculty of Engineering, Chiang Mai University, Chiang Mai, 50200, Thailand.
Biomedical Engineering Institute, Chiang Mai University, Chiang Mai, Thailand.
Ann Biomed Eng. 2020 Jun;48(6):1702-1711. doi: 10.1007/s10439-020-02487-z. Epub 2020 Feb 26.
We demonstrated the use of multispectral cryo-imaging and software to analyze human mesenchymal stromal cells (hMSCs) biodistribution in mouse models of graft-versus-host-disease (GVHD) following allogeneic bone marrow transplantation (BMT). We injected quantum dot labeled MSCs via tail vein to mice receiving BMT and analyzed hMSC biodistribution in major organs (e.g. lung, liver, spleen, kidneys and bone marrow). We compared the biodistribution of hMSCs in mice following allogeneic BMT recipients (with GVHD) to the biodistribution following syngeneic BMT (without GVHD). Cryo-imaging system revealed cellular biodistribution and redistribution patterns in the animal model. We initially found clusters of cells in the lung that eventually dissociated to single cells and redistributed to other organs within 72 h. The in vivo half-life of the exogenous MSCs was about 21 h. We found that the biodistribution of stromal cells was not related to blood flow, rather cells preferentially homed to specific organs. In conclusion, cryo-imaging was suitable for analyzing the cellular biodistribution. It could provide capabilities of visualizing cells anywhere in the mouse model with single cell sensitivity. By characterizing the biodistribution and anatomical specificity of a therapeutic cellular product, we believe that cryo-imaging can play an important role in the advancement of stem and stromal cell therapies and regenerative medicine.
我们展示了使用多光谱冷冻成像和软件来分析同种异体骨髓移植（BMT）后移植物抗宿主病（GVHD）小鼠模型中人间充质基质细胞（hMSC）的生物分布。我们通过尾静脉注射量子点标记的 MSC 到接受 BMT 的小鼠中，并分析 hMSC 在主要器官（如肺、肝、脾、肾和骨髓）中的生物分布。我们比较了同种异体 BMT 受者（有 GVHD）和同基因 BMT（无 GVHD）后 hMSC 的生物分布。冷冻成像系统揭示了动物模型中细胞的生物分布和再分布模式。我们最初在肺中发现了细胞簇，这些细胞最终在 72 小时内分离成单个细胞并重新分布到其他器官。外源性 MSC 的体内半衰期约为 21 小时。我们发现基质细胞的生物分布与血流无关，而是细胞优先归巢到特定的器官。总之，冷冻成像适用于分析细胞的生物分布。它可以提供在小鼠模型中以单细胞灵敏度可视化任何部位细胞的能力。通过表征治疗性细胞产品的生物分布和解剖学特异性，我们相信冷冻成像可以在干细胞和基质细胞治疗和再生医学的发展中发挥重要作用。
