Key Laboratory of Pollution Ecology and Environmental Engineering, Institute of Applied Ecology, Chinese Academy of Sciences, Shenyang, 110164, P. R. China.
Graduate University of Chinese Academy of Sciences, Beijing, 100049, P. R. China.
J Microbiol. 2020 Apr;58(4):279-287. doi: 10.1007/s12275-020-9409-8. Epub 2020 Feb 27.
Little is known about the distribution of fungal communities with soil depth on relatively large scales. In this study, typical paddy soils in three regions (Hailun, Changshu, and Yingtan) from north to south China were selected to investigate the vertical distribution (0-100 cm) of the fungal community by Illumina MiSeq sequencing, and to identify the main factors influencing the fungal community distribution. The results indicated that the structure of the soil fungal community changed significantly with region and soil depth. Soil fungal taxa such as Zygomycota, Glomeromycota, Saccharomycete, Kazachstania, Mortierella, Massariosphaeria, Hypholoma, and Zopfiella were enriched at depths of 0-20 cm, whereas Dothideomycetes, Microbotryomycetes, Tremellomycetes, Sporobolomyces, Cryptococcus, Rhodotorula, Fusarium, and Pyrenochaetopsis had high relative abundances at 80-100 cm. Variance partitioning analysis indicated that the geographic distance contributed more to the fungal community variation than environmental variables on a large scale. In addition, soil total carbon and nitrogen contents were the main environmental factors driving the vertical distribution of the fungal community in paddy soils.
关于真菌群落随土壤深度在较大尺度上的分布情况，目前知之甚少。本研究选择了中国从北到南的三个地区（海伦、常熟和鹰潭）的典型稻田土壤，通过 Illumina MiSeq 测序来研究真菌群落的垂直分布（0-100cm），并确定影响真菌群落分布的主要因素。结果表明，土壤真菌群落的结构随地区和土壤深度的变化而显著变化。土壤真菌类群如接合菌门、球囊霉门、酵母纲、Kazachstania、肉座菌属、Massariosphaeria 属、丝盖伞属和 Zopfiella 属在 0-20cm 深度处富集，而子囊菌门、小丛壳菌门、酵母纲、Sporobolomyces 属、隐球菌属、红酵母属、镰刀菌属和 Pyrenochaetopsis 属在 80-100cm 深度处相对丰度较高。方差分解分析表明，在较大尺度上，地理距离对真菌群落变异的贡献大于环境变量。此外，土壤总碳和氮含量是驱动稻田土壤真菌群落垂直分布的主要环境因素。
