Yang Jinsong, Chen Wei, Xia Pinchang, Zhang Wenchang
Fujian Provincial Key Laboratory of Environment Factors and Cancer, Key Laboratory of Environment and Health, School of Public Health, Fujian Medical University, Fuzhou, Fujian 350122, P.R. China.
Department for Viral Disease Control and Prevention, Fujian Center for Disease Control and Prevention, Fuzhou, Fujian 350001, P.R. China.
Exp Ther Med. 2020 Mar;19(3):2273-2281. doi: 10.3892/etm.2020.8469. Epub 2020 Jan 23.
is one of the main pathogens causing diarrheal disease, and is associated with high morbidity and mortality in developing countries. Previous clinical data and animal studies have shown that the outcomes of oral and peritoneal infections of differ, and that the latter is more serious. Furthermore, a variety of pathogenic bacteria are known to cause changes in intestinal flora after infection, and the influence of infection on intestinal flora remains poorly understood. In the present study, the 16S rRNA high-throughput sequencing method was used to compare the changes in gut microbiota profiles in feces of mice infected with via two routes. In addition, the present study investigated the association between the differences in infection performance and bacterial communities. The present results suggested that the intraperitoneal route induced a distinct decrease in α-diversity in the fecal microbiota when compared to the control at a later time, while the effect of the oral route on α-diversity was not obvious. Oral infection of had a rapid and significant effect on gut microbiota, mainly causing a decreased abundance of and an increased abundance of and in the early stage of infection. By contrast, the effect of intraperitoneal infection on the gut microbiota was relatively slow and small. The principal coordinate analysis results suggested that the dynamic profile of gut microbiota between the two infective routes was consistent with the infection process. Probiotics, such as and exhibited significantly reduced abundance after infection. Collectively, the present results suggested that gut microbiota may play a pivotal role in the pathogenesis of infection. Future studies should investigate the effect of infection on the interaction between pathogenic bacteria and intestinal flora. The present results suggested that the use of probiotics may facilitate the prevention and treatment of shigellosis.
是引起腹泻病的主要病原体之一，在发展中国家与高发病率和高死亡率相关。先前的临床数据和动物研究表明，口服感染和腹腔感染的结果不同，且后者更严重。此外，已知多种病原菌感染后会导致肠道菌群发生变化，但痢疾杆菌感染对肠道菌群的影响仍知之甚少。在本研究中，采用16S rRNA高通量测序方法比较了经两种途径感染痢疾杆菌的小鼠粪便中肠道微生物群谱的变化。此外，本研究还调查了感染表现差异与细菌群落之间的关联。目前的结果表明，与对照组相比，腹腔途径在后期导致粪便微生物群的α多样性明显降低，而口服途径对α多样性的影响不明显。口服痢疾杆菌感染对肠道微生物群有快速且显著的影响，主要在感染早期导致大肠杆菌丰度降低，以及双歧杆菌和乳酸杆菌丰度增加。相比之下，腹腔感染对肠道微生物群的影响相对缓慢且较小。主坐标分析结果表明，两种感染途径之间肠道微生物群的动态谱与感染过程一致。益生菌，如双歧杆菌和乳酸杆菌，在感染痢疾杆菌后丰度显著降低。总体而言，目前的结果表明肠道微生物群可能在痢疾杆菌感染的发病机制中起关键作用。未来的研究应调查痢疾杆菌感染对病原菌与肠道菌群相互作用的影响。目前的结果表明，使用益生菌可能有助于预防和治疗志贺氏菌病。
