Liu Jiaxin, Yang Xueyan, Li Lin, Zhang Qili, Zhang Zhaoyan, Zhang Xin, Zhao Yunli, Yu Miao, Yu Zhiguo
Shenyang Pharmaceutical University, No.103, Wenhua Road, Shenyang 110016, China.
Asian J Pharm Sci. 2017 Jul;12(4):363-369. doi: 10.1016/j.ajps.2016.09.001. Epub 2016 Sep 22.
A rapid, simple and sensitive ultra-high performance liquid chromatography-tandem mass spectrometric (UPLC-MS/MS) method was developed and validated for the determination of hederasaponin B, an active triterpenoid saponin widely existed in L. Plasma samples were processed by protein precipitation with acetonitrile and separated on a Thermo Hypersil GOLD C (2.1 mm × 50 mm,1.9 µm) at flow rate of 0.3 ml/min, with a gradient elution consisting of acetonitrile and water containing 0.1% (v/v) formic acid at 30 °C and detected by electrospray ionization mass spectrometry in the positive multiple reaction monitoring (MRM) mode. The linearity was found to be within the concentration range of 0.5-5000 ng/ml with a lower limit of quantification of 0.5 ng/ml. The absolute oral bioavailability of hederasaponin B was 0.24 ± 0.49%. This indicated that the concentration-time course of the hederasaponin B existed a double-peak phenomenon. This method was further applied to the determination of hederasaponin B in rat plasma and showed good practicability, for the first time, after intragastric (25 mg/kg) and intravenous (2 mg/kg) administration in rats.
建立并验证了一种快速、简便、灵敏的超高效液相色谱-串联质谱法（UPLC-MS/MS），用于测定常春藤皂苷B，一种广泛存在于[植物名称未给出]中的活性三萜皂苷。血浆样品通过乙腈蛋白沉淀法处理，在Thermo Hypersil GOLD C（2.1 mm × 50 mm，1.9 µm）柱上以0.3 ml/min的流速分离，梯度洗脱由乙腈和含0.1%（v/v）甲酸的水组成，在30 °C下进行，通过电喷雾电离质谱在正离子多反应监测（MRM）模式下检测。线性范围为0.5 - 5000 ng/ml，定量下限为0.5 ng/ml。常春藤皂苷B的绝对口服生物利用度为0.24 ± 0.49%。这表明常春藤皂苷B的浓度-时间过程存在双峰现象。该方法首次应用于大鼠血浆中常春藤皂苷B的测定，在大鼠灌胃（25 mg/kg）和静脉注射（2 mg/kg）给药后显示出良好的实用性。
