Schwertschlag U S, Dennis V W, Tucker J A, Camussi G
Department of Medicine, Duke University Medical Center, Durham, North Carolina.
Kidney Int. 1988 Dec;34(6):779-85. doi: 10.1038/ki.1988.249.
Rat kidneys were isolated and perfused with a cell-free perfusion buffer containing 4% albumin. Infusion of platelet activating factor (s-PAF) into the isolated perfused kidney caused a dose-dependent fall in renal vascular resistance (RVR): 12 +/- 6% at 10 nM s-PAF, 18 +/- 3% at 100 nM s-PAF and 20 +/- 7% at 1 microM s-PAF. Glomerular filtration rate fell by 32 +/- 5% at 10 nM, 38 +/- 6% at 100 nM, and 52 +/- 10% at 1 microM. s-PAF (50 nM) increased urinary protein excretion after 20 minutes. Because GFR fell to a greater extent than RVR, possible changes in glomerular permeability after s-PAF treatment were assessed morphologically using native ferritin. After s-PAF treatment (100 nM), the number of ferritin particles/micron2 increased from 1.2 +/- 0.9 (control) to 795 +/- 69 in the glomerular basement membrane (GBM) and from 0.2 +/- 0.06 (control) to 98 +/- 29 in lamina rara externa (LRE). To quantitate changes in fixed anionic charges, polyethylenimine (PEI) was quantitated morphologically in GBM. No significant change between s-PAF treated and untreated kidneys was seen. s-PAF did not alter the sialoglycoprotein pattern in the perfused kidney as assessed by lysozyme staining. These results are in contrast to findings with s-PAF in vivo where in addition to increased glomerular permeability, a reduction of fixed anionic charges is seen.(ABSTRACT TRUNCATED AT 250 WORDS)
分离大鼠肾脏，并用含4%白蛋白的无细胞灌注缓冲液进行灌注。向分离的灌注肾中输注血小板活化因子（s-PAF）可导致肾血管阻力（RVR）呈剂量依赖性下降：10 nM s-PAF时下降12±6%，100 nM s-PAF时下降18±3%，1 μM s-PAF时下降20±7%。肾小球滤过率在10 nM时下降32±5%，100 nM时下降38±6%，1 μM时下降52±10%。s-PAF（50 nM）在20分钟后增加尿蛋白排泄。由于肾小球滤过率下降幅度大于肾血管阻力，因此使用天然铁蛋白从形态学上评估s-PAF处理后肾小球通透性的可能变化。s-PAF处理（100 nM）后，肾小球基底膜（GBM）中铁蛋白颗粒数/μm²从1.2±0.9（对照）增加到795±69，在外侧稀疏层（LRE）中从0.2±0.06（对照）增加到98±29。为了定量固定阴离子电荷的变化，在GBM中从形态学上对聚乙烯亚胺（PEI）进行定量。s-PAF处理组和未处理组肾脏之间未见显著变化。通过溶菌酶染色评估，s-PAF未改变灌注肾中的唾液糖蛋白模式。这些结果与s-PAF在体内的研究结果形成对比，在体内除了肾小球通透性增加外，还可见固定阴离子电荷减少。（摘要截断于250字）
