School of Biomedical Engineering, Beijing University of Technology, Beijing, China.
Laser Institute, Beijing University of Technology, Beijing, China.
Int J Neurosci. 2021 Jan;131(1):40-43. doi: 10.1080/00207454.2020.1733553. Epub 2020 Feb 28.
The study aims to lay a foundational probe for the thorough application microfluidic chips in brain function research with microfluidic chips. Neuron slide culture is a common culture method , and the microfluidic chip with the artificial network pattern not only can realize neuron cells 3 D culture , but also limit the extension space of neurite outgrow. In order to analyze the differences of hippocampal cells neurite growth length between the 3 D chips and the common 2 D culture, the experiments utilized statistical analysis method analyzing the length of the hippocampus neuron neurite of 3 days, 5 days and 7 days, respectively, with the common glass slide 2 D culture method and the microfluidic chip 3 D culture . The results showed that there was no significant difference in the neurite length after 3 days. However, there was a significant difference after 5 days and 7 days. It can be seen that the microfluidic chip with artificial network pattern has limitations to the growth of neurite after 5 days. We concluded that the growth state of hippocampal cells in the restricted 3 D space is different from that of conventional 2 D culture.It showed that the artificial network pattern design has limited the growth space of the dendrites but also affected its growth.
本研究旨在为微流控芯片在脑功能研究中的深入应用奠定基础。神经元滑行培养是一种常见的培养方法，而具有人工网络图案的微流控芯片不仅可以实现神经元细胞的 3D 培养，还可以限制神经突生长的扩展空间。为了分析 3D 芯片与普通 2D 培养中海马细胞神经突生长长度的差异，实验分别采用统计分析方法分析了普通玻璃载玻片 2D 培养法和微流控芯片 3D 培养法培养 3 天、5 天和 7 天的海马神经元神经突的长度。结果表明，培养 3 天后神经突长度没有显著差异。然而，培养 5 天后和 7 天后有显著差异。可以看出，人工网络图案的微流控芯片对 5 天后神经突的生长有一定的限制。我们得出结论，在受限的 3D 空间中，海马细胞的生长状态与传统的 2D 培养不同。这表明人工网络图案设计限制了树突的生长空间，也影响了其生长。
