Liaoning Provincial Key Laboratory of Zoonosis, College of Animal Science and Veterinary Medicine, Shenyang Agricultural University, Shenyang, 110866, Liaoning, People's Republic of China.
Amino Acids. 2020 Mar;52(3):453-463. doi: 10.1007/s00726-020-02824-5. Epub 2020 Feb 27.
The calpain-1-activated apoptotic pathway plays a key role in right ventricular hypertrophy (RVH). Taurine has been shown to attenuate apoptosis by inhibiting calpain activity. This experiment aimed to determine whether taurine could prevent RVH by inhibiting the calpain-1/cytochrome c apoptotic pathway. The broilers were given 1% taurine dissolved in drinking water and were raised at 10 °C ~ 12 °C from day 21 to day 42. At 21 d, 28 d, 35 d and 42 d, the right ventricular (RV) tissues were collected. Increased RVH index, angiotensin II, norepinephrine and atrial natriuretic peptide mRNA expression were reduced by taurine in the broiler RVs. Taurine obviously inhibited cardiomyocyte apoptosis via maintaining the mitochondrial membrane potential and decreased the activation of caspase-9 and caspase-3 in the broiler RVs. The antioxidant assay demonstrated that taurine enhanced the activities of superoxide dismutase, total antioxidant capacity and glutathione peroxidase and the glutathione/glutathione disulfide ratio. Western blot results revealed that taurine also downregulated the expression of calpain-1 and cytosolic cytochrome c while upregulating the expression of Bcl-2/Bax and mitochondrial cytochrome c in broiler cardiomyocytes during RVH. In summary, we found that taurine could enhance cardiomyocyte antioxidant ability and further prevented cardiomyocyte apoptosis by inhibiting the calpain-1/cytochrome c pathway during RVH in broilers.
钙蛋白酶-1 激活的凋亡途径在右心室肥厚（RVH）中发挥关键作用。牛磺酸已被证明通过抑制钙蛋白酶活性来减轻细胞凋亡。本实验旨在确定牛磺酸是否可以通过抑制钙蛋白酶-1/细胞色素 c 凋亡途径来预防 RVH。肉鸡从第 21 天到第 42 天，给予 1%溶于饮用水中的牛磺酸，并在 10°C 至 12°C 下饲养。在第 21、28、35 和 42 天，收集右心室（RV）组织。牛磺酸可降低肉鸡 RV 中的右心室肥厚指数、血管紧张素 II、去甲肾上腺素和心钠肽 mRNA 表达。牛磺酸通过维持线粒体膜电位，明显抑制肉鸡 RV 中的心肌细胞凋亡，并降低 caspase-9 和 caspase-3 的激活。抗氧化测定表明，牛磺酸增强了超氧化物歧化酶、总抗氧化能力和谷胱甘肽过氧化物酶的活性，并增加了谷胱甘肽/谷胱甘肽二硫化物的比值。Western blot 结果表明，牛磺酸还下调了钙蛋白酶-1 和胞浆细胞色素 c 的表达，同时上调了肉鸡心肌细胞中 Bcl-2/Bax 和线粒体细胞色素 c 的表达。综上所述，我们发现牛磺酸可以通过抑制钙蛋白酶-1/细胞色素 c 途径增强心肌细胞的抗氧化能力，进一步预防 RVH 中的心肌细胞凋亡。
