丙型肝炎病毒 NS5A 的结构域 I 以基因型依赖的方式与 ACBD3 相关联。

Domain I of hepatitis C virus NS5A associates with ACBD3 in a genotype-dependent manner.

机构信息

School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, Shandong, China.

Institute of Basic Medicine, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, China.

出版信息

Microbiol Immunol. 2020 Aug;64(8):574-577. doi: 10.1111/1348-0421.12784. Epub 2020 Jul 3.

DOI:10.1111/1348-0421.12784

PMID:32108375

Abstract

Previously, it was found that the hepatitis C virus NS5A interacted with ACBD3 in a genotype-dependent manner. However, the region in NS5A responsible for association with ACBD3 is not clear. Domain I of NS5A was identified as critical for ACBD3 binding. By comparing the differences of amino acids in domain I from different genotypes of NS5A, it was found that key amino acids potentially corresponded to the affinity of the NS5A-ACBD3 interaction. The findings not only revealed that domain I of NS5A associates with ACBD3 but they also shed mechanistic light on how NS5A is associated with ACBD3 in a genotype-dependent manner.

摘要

先前发现丙型肝炎病毒 NS5A 以基因型依赖的方式与 ACBD3 相互作用。然而，NS5A 中与 ACBD3 结合的区域尚不清楚。NS5A 的结构域 I 被确定为与 ACBD3 结合的关键区域。通过比较不同基因型 NS5A 中结构域 I 氨基酸的差异，发现关键氨基酸可能与 NS5A-ACBD3 相互作用的亲和力相对应。这些发现不仅揭示了 NS5A 的结构域 I 与 ACBD3 相关，而且还阐明了 NS5A 以基因型依赖的方式与 ACBD3 相互作用的机制。