Fisheries Science Institute, Chonnam National University, Yeosu, South Korea.
Department of Animal Health and Management, Alagappa University, Karaikudi, Tamil Nadu, India.
Microsc Res Tech. 2020 Jul;83(7):736-743. doi: 10.1002/jemt.23463. Epub 2020 Feb 28.
In the present study, Microscopy studies were performed to characterize the blood cells of the mangrove crab Episesarma tetragonum. Three types of hemocytes were observed: granulocytes, semi-granulocytes, and hyalinocytes or agranulocytes. Hyalinocytes have a distinguished nucleus surrounded by the cytoplasm, and a peculiar cell type was present throughout the cytosol, lysosomes with hemocyte types (granules) stained red (pink). Giemsa staining was used to differentiate between the large and small hemocytes. Ehrlich's staining was used to differentiate granule-containing cells in acidophils (55%), basophils (44%), and neutrophils (<1%). Periodic acid-Schiff staining was used to identify the sugar molecules in the cytoplasm. Cell-mediated immune reactions including phagocytosis, encapsulation, agglutination, and peroxidase-mediated cell adhesion are the functions of hemocytes. Agglutination reaction involves both kind of cells involved in yeast and heme-agglutination responses in invertebrates. The beta glucan outer layer of yeast cells was recognized by hemocyte receptors. Human RBC cells were agglutinated via granulocytes. E. tetragonum hemocytes are an important animal model for studying both ultrastructural and functional activity of circulating cells. In addition, E. tetragonum hemocytes exhibited excellent antibacterial and antibiofilm activities were studied through plating and microplate assays. Biofilm inhibition was also visualized through changes in biochemical assays and morphological variations were visualized through levels in in situ microscopy analysis.
在本研究中，进行了显微镜研究以表征红树林蟹 Episesarma tetragonum 的血细胞。观察到三种类型的血细胞：粒细胞、半粒细胞和透明细胞或无粒细胞。透明细胞具有被细胞质包围的独特细胞核，细胞质中存在一种特殊的细胞类型，溶酶体与血细胞类型（颗粒）染成红色（粉红色）。吉姆萨染色用于区分大、小血细胞。Ehrlich 染色用于区分含颗粒的细胞，嗜酸粒细胞（55%）、嗜碱性粒细胞（44%）和中性粒细胞（<1%）。过碘酸雪夫染色用于鉴定细胞质中的糖分子。细胞介导的免疫反应包括吞噬作用、包被作用、凝集作用和过氧化物酶介导的细胞黏附作用，这些都是血细胞的功能。凝集反应涉及到参与酵母和无脊椎动物血红素凝集反应的两种类型的细胞。酵母细胞的β葡聚糖外层被血细胞受体识别。人类 RBC 细胞通过粒细胞凝集。E. tetragonum 血细胞是研究循环细胞的超微结构和功能活性的重要动物模型。此外，通过平板和微量板测定研究了 E. tetragonum 血细胞的出色的抗菌和抗生物膜活性。通过生化测定的变化可视化生物膜抑制，通过原位显微镜分析水平可视化形态变化。
