Heerschap A, van Vaals J J, Bergman A H, den Boef J H, van Gerwen P H, Gravenmade E J
Philips Research Laboratories, Eindhoven, The Netherlands.
Magn Reson Med. 1988 Oct;8(2):129-41. doi: 10.1002/mrm.1910080203.
In vivo 31P NMR spectra of rat submandibular glands were obtained. The glands were exposed, leaving the neurovascular system intact, and placed in a solenoidal coil. Resonances of nine phosphate metabolites were identified in the spectra and metabolite concentrations were estimated from the corresponding integrals ([ATP] = 3.4 +/- 0.7 mM). Tissue pH, as deduced from the chemical shift of the inorganic phosphate resonance, was 7.26 (+/- 0.07). T1 relaxation times of ATP, phosphocreatine, inorganic phosphate, and phosphomonoester 31P spin systems were examined. The effect of hypoxia was followed as a function of time. 31P NMR spectra of the glands have also been obtained noninvasively by the use of a surface coil, adapted to the dimensions of the glands, and depth pulses.
获得了大鼠下颌下腺的体内31P NMR谱。暴露腺体，使神经血管系统保持完整，然后将其置于螺线管线圈中。在谱中鉴定出九种磷酸代谢物的共振峰，并根据相应的积分估计代谢物浓度（[ATP]=3.4±0.7 mM）。根据无机磷酸共振的化学位移推断，组织pH值为7.26（±0.07）。检测了ATP、磷酸肌酸、无机磷酸和磷酸单酯31P自旋系统的T1弛豫时间。观察了缺氧效应随时间的变化。还通过使用适合腺体尺寸的表面线圈和深度脉冲，以非侵入性方式获得了腺体的31P NMR谱。
