Schlatt Lukas, Costa Ana Carolina Conchon, Barz Vivien, Ciarimboli Giuliano, Karst Uwe
University of Münster, Institute of Inorganic and Analytical Chemistry, Corrensstraße 28/30, 48149 Münster, Germany.
University Hospital Münster, Experimental Nephrology, Medicine Clinic D, Albert-Schweitzer-Campus 1/ A14, 48149 Münster, Germany.
J Pharm Biomed Anal. 2020 May 30;184:113172. doi: 10.1016/j.jpba.2020.113172. Epub 2020 Feb 13.
A high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) method was successfully developed for the simultaneous detection of gabapentin (GBP) and cetirizine (CTZ) in cell lysates. Multiple reaction monitoring (MRM) transitions were found for GBP, CTZ and deuterated internal standards of both, allowing a selective and sensitive detection. Limits of detection (LODs) of 0.04 ng/mL and 0.07 ng/mL were achieved for CTZ and GBP respectively, and a linear range could be confirmed to the highest tested concentration of 50 ng/mL. Furthermore, with a total runtime of 2.5 min, this method allows a high throughput analysis. The applicability of the method was demonstrated by using it for the quantification of CTZ and GBP in cell lysates to examine the effects of renal transporters on these two analytes.
成功开发了一种高效液相色谱-串联质谱（HPLC-MS/MS）方法，用于同时检测细胞裂解物中的加巴喷丁（GBP）和西替利嗪（CTZ）。发现了GBP、CTZ及其二者氘代内标的多反应监测（MRM）转换，实现了选择性和灵敏检测。CTZ和GBP的检测限（LOD）分别为0.04 ng/mL和0.07 ng/mL，并且可以确认线性范围至最高测试浓度50 ng/mL。此外，该方法总运行时间为2.5分钟，可实现高通量分析。通过将该方法用于定量细胞裂解物中的CTZ和GBP以检查肾转运体对这两种分析物的影响，证明了该方法的适用性。
