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[产多烯抗生素玫红菌素的不同变异型玫瑰色放线菌的比较研究]

[Comparative study of different variants of Actinomyces roseoflavus producing the polyene antibiotic roseofungin].

作者信息

Korotkova T P, Faĭ M E, Zhukova I G, Nikitina E T, Sheĭko T V

出版信息

Mikrobiologiia. 1988 Jul-Aug;57(4):642-7.

PMID:3211008
Abstract

The respiration chain in the membranes of whole Actinomyces roseoflavus (var. roseofungini) cells from the parent and secondary cultures is sensitive to KCN, non-sensitive to Triton X-100 treatment removing the antibiotic roseofungin from the cells, and has a very high for the bacteria respiration control. When the cells are in contact with atomic tritium at the temperature of liquid nitrogen, roseofungin is tritiated and binds to A. roseoflavus isolated membranes and whole cells, mostly to those of the parent culture as compared to the secondary culture. A fraction of membranes which lost NADH dehydrogenase in the course++ of purification was isolated from the cells disintegrated in the frozen state.

摘要

来自亲代培养物和继代培养物的全玫瑰黄链霉菌(玫瑰真菌变种)细胞的膜中的呼吸链对氰化钾敏感,对用Triton X-100处理从细胞中去除抗生素玫瑰真菌素有抗性,并且对细菌呼吸控制具有非常高的抗性。当细胞在液氮温度下与氚化原子接触时,玫瑰真菌素被氚化并与分离的玫瑰黄链霉菌膜和全细胞结合,与继代培养物相比,主要与亲代培养物的膜和细胞结合。从冷冻状态下破碎的细胞中分离出一部分在纯化过程中失去NADH脱氢酶的膜。

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1
[Comparative study of different variants of Actinomyces roseoflavus producing the polyene antibiotic roseofungin].[产多烯抗生素玫红菌素的不同变异型玫瑰色放线菌的比较研究]
Mikrobiologiia. 1988 Jul-Aug;57(4):642-7.
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