Gilbert M E
Northrop Services Inc., Research Triangle Park, NC 27709.
Neurotoxicol Teratol. 1988 May-Jun;10(3):221-7. doi: 10.1016/0892-0362(88)90021-9.
Electrical kindling of the amygdala and hippocampus was used to evaluate the effects of two formamidines, chlordimeform (CDF) and amitraz (AMZ), upon seizures susceptibility in the rat. Male Long-Evans rats were implanted with electrodes in the amygdala or dorsal dentate gyrus, and injected IP daily with 40 mg/kg CDF, 50 mg/kg AMZ, or equal volumes of their respective vehicles. Afterdischarge (AD) thresholds were determined after the first injection. Animals were then stimulated twice daily, 2 and 4 hours postinjeciton, at a standard 200 microA stimulus intensity until three stage 5 generalized seizures ensued. Both CDF and AMZ significantly facilitated amygdaloid kindling rate, and CDF also facilitated hippocampal kindling rate. The effects of AMZ on hippocampal kindling were not assessed. AD durations were prolonged in the formamidine-treated groups, but there was no effect on AD thresholds. The alpha-2 adrenergic agonist and/or local anesthetic-like properties of these compounds may be responsible for these seizure enhancing effects.
用电刺激杏仁核和海马体的方法来评估两种脒类化合物，即杀虫脒（CDF）和双甲脒（AMZ）对大鼠癫痫易感性的影响。将雄性Long-Evans大鼠在杏仁核或齿状回背侧植入电极，每天腹腔注射40mg/kg CDF、50mg/kg AMZ或等量的各自溶剂。首次注射后测定后放电（AD）阈值。然后每天在注射后2小时和4小时以标准的200微安刺激强度对动物进行两次刺激，直到出现三次5期全身性癫痫发作。CDF和AMZ均显著促进杏仁核点燃率，CDF还促进海马体点燃率。未评估AMZ对海马体点燃的影响。在脒类化合物处理组中，AD持续时间延长，但对AD阈值没有影响。这些化合物的α-2肾上腺素能激动剂和/或局部麻醉样特性可能是这些癫痫增强作用的原因。
