Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India.
Department of Biochemistry and Biotechnology, Annamalai University, Annamalainagar, Tamilnadu, India.
Arch Med Res. 2020 Apr;51(3):204-214. doi: 10.1016/j.arcmed.2020.02.001. Epub 2020 Feb 26.
Oxidative and endoplasmic reticulum stresses contribute to the pathogenesis of β-cell dysfunction in diabetes mellitus. This study investigates the effect of isopulegol on the above stresses in HFD/STZ induced diabetic rats.
Animals in group I and II were placed in normal pellet diet and group II was treated with isopulegol at 200 mg/kg b.w. Animals in groups III-V were placed in HFD for 4 weeks and made diabetic with single intraperitoneal injection of STZ (35 mg/kg b.w) in 0.1 M citrate buffer (pH 4.5). Group III served as diabetic control while animals in group IV and V were treated with isopulegol (100 mg/kg b.w) and metformin (25 mg/kg b.w) respectively for 28 d.
The activities of superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione sulphur transferase (GST), glutathione reductase (GR) and the levels of vitamin-E, vitamin-C, reduced glutathione (GSH) were significantly (p <0.05) decreased in plasma and tissues of diabetic rats. Thiobarbituric acid reactive acid substances (TBARS) and lipid hydroperoxides (LHP), indices of lipid peroxidation were also significantly (p <0.05) increased in diabetic rats. In pancreatic tissue ER stress markers PERK, elf2α, ATF4 and in hepatic tissue oxidative stress marker UCP-2 expression was significantly (p <1.0) increased in diabetic rats. Administration of isopulegol significantly improved antioxidant status and decreased oxidative and ER stress markers in diabetic treated rats. Histopathological studies on liver and kidney supported the above findings. The results are comparable with the standard drug metformin.
Isopulegol a naturally occurring monoterpene alcohol attenuated oxidative and ER stress in HFD/STZ induced diabetic rats.
氧化应激和内质网应激导致糖尿病β细胞功能障碍。本研究探讨了异胡薄荷醇对 HFD/STZ 诱导的糖尿病大鼠上述应激的影响。
I 组和 II 组动物给予普通颗粒饮食,II 组给予异胡薄荷醇 200mg/kg 体重。III-V 组动物给予高脂肪饮食 4 周,用 0.1M 柠檬酸盐缓冲液(pH4.5)中的 STZ(35mg/kg 体重)单次腹腔注射诱导糖尿病。III 组为糖尿病对照组,IV 组和 V 组分别给予异胡薄荷醇(100mg/kg 体重)和二甲双胍(25mg/kg 体重)治疗 28 天。
糖尿病大鼠血浆和组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、谷胱甘肽过氧化物酶(GPx)、谷胱甘肽硫转移酶(GST)、谷胱甘肽还原酶(GR)的活性以及维生素-E、维生素-C、还原型谷胱甘肽(GSH)的水平显著降低(p<0.05)。丙二醛(TBARS)和脂质过氧化物(LHP),脂质过氧化的指标也显著升高(p<0.05)。糖尿病大鼠胰腺组织中 PERK、elf2α、ATF4 等内质网应激标志物和肝组织中 UCP-2 等氧化应激标志物的表达显著增加(p<0.05)。异胡薄荷醇治疗可显著改善糖尿病大鼠的抗氧化状态,降低氧化应激和内质网应激标志物。肝、肾组织学研究支持上述发现。这些结果与标准药物二甲双胍相当。
天然单萜醇异胡薄荷醇可减轻 HFD/STZ 诱导的糖尿病大鼠的氧化应激和内质网应激。